Molecular Characterization of a Cytosolic Malate Dehydrogenase Gene(GhcMDH1) from Cotton

doi:10.1007/s40242-017-6358-9

Chemical Research in Chinese Universities ›› 2017, Vol. 33 ›› Issue (1): 87-93.doi: 10.1007/s40242-017-6358-9

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Molecular Characterization of a Cytosolic Malate Dehydrogenase Gene(GhcMDH1) from Cotton

IMRAN Muhammad¹, ZHANG Bing^1,2, TANG Kai¹, LIU Jinyuan¹

1. Laboratory of Plant Molecular Biology, Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, P. R. China;
2. Institute of Botany, Chinese Academy of Sciences, Nanjing 210014, P. R. China

Received:2016-08-30 Revised:2016-11-14 Online:2017-02-01 Published:2016-11-28
Contact: LIU Jinyuan,E-mail:liujy@mail.tsinghua.edu.cn E-mail:liujy@mail.tsinghua.edu.cn
Supported by:
Supported by the National Transgenic Animals and Plants Research Project, China(Nos.2011ZX08005-003, 2011ZX08009-003).

Abstract

Abstract:

Malate dehydrogenase(MDH) is a key enzyme that catalyzes the reversible oxidation of oxaloacetate to malate and plays an important role in the physiological processes of plant growth and development. However, cyt osolic malate dehydrogenase(cMDH), which is crucial for malate synthesis in the cytosol, still has not been extensively characterized in plants. Here, we isolated a cytosolic malate dehydrogenase gene, designated as GhcMDH1, from Gossypium hirsutum and characterized its possible molecular function in cotton fiber. The cloned cDNA of GhcMDH1 is 1520 base pairs in length, and has an open reading frame of 999 base pairs, encoding for 332 amino acid residues with an estimated molecular weight of 35580 and pI of 6.35. Sequence alignment showed that the deduced amino acid sequence of GhcMDH1 protein shared a high similarity to other plant cMDHs. Confocal and immunological analysis confirmed that GhcMDH1 protein was subcellularly localized to the cytosol. Quantitative real-time polymerase chain reaction(PCR) revealed that GhcMDH1 was constitutively expressed in all vegetative cotton tissues, with slightly lower levels in roots than stems and leaves. Interestingly, the transcripts of GhcMDH1 were detected in 5-25 d post anthesis(DPA) fibers and highly abundant at 15 DPA fibers. The total MDH activities and malate contents of cotton fibers were positively correlated with the fiber elongation rates, suggesting that GhcMDH1 may function in malate synthesis in fast fiber elongation. In agreement with this suspicion, the recombinant His-GhcMDH1 protein mainly drives the reaction towards malate generation in vitro. In conclusion, our molecular characterization of the GhcMDH1 gene provides valuable insights to further investigate the malate equilibrium in cotton fiber development.

Key words: Cotton, Cytosolic malate dehydrogenase, Malate synthesis, Fiber development

IMRAN Muhammad, ZHANG Bing, TANG Kai, LIU Jinyuan. Molecular Characterization of a Cytosolic Malate Dehydrogenase Gene(GhcMDH1) from Cotton[J]. Chemical Research in Chinese Universities, 2017, 33(1): 87-93.

References

[1] Kim H. J., Triplett B. A., Plant Physiol., 2001, 127(4), 1361
[2] Lee J. J., Woodward A. W., Chen Z. J., Ann. Bot., 2007, 100(7), 1391
[3] Qin Y. M., Zhu Y. X., Curr. Opin. Plant Biol., 2011, 14(1), 106
[4] Cosgrove D. J., The Plant Cell, 1997, 9(7), 1031
[5] Kim H. J., Triplett B. A., Plant Physiol., 2001, 127(4), 1361
[6] Ruan Y. L., Llewellyn D. J., Furbank R. T., The Plant cell, 2001, 13(1), 47
[7] Ruan Y. L., Funct. Plant Biol., 2007, 34(1), 1
[8] Smart L. B., Vojdani F., Maeshima M., Wilkins T. A., Plant Physiol., 1998, 116(4), 1539
[9] Arpat A. B., Waugh M., Sullivan J. P., Gonzales M., Frisch D., Main D., Wood T., Leslie A., Wing R. A., Wilkins T. A., Plant Mol. Biol., 2004, 54(6), 911
[10] Kumar S., Kumar K., Pandey P., Rajamani V., Padmalatha K. V., Dhandapani G., Kanakachari M., Leelavathi S., Kumar P. A., Reddy V. S., Mol. Cell. Proteomics, 2013, 12(12), 3677
[11] Thaker V., Rabadia V., Singh Y., Acta Physiol. Plant, 1999, 21(1), 57
[12] Pang C. Y., Wang H., Pang Y., Xu C., Jiao Y., Qin Y. M., Western T. L., Yu S. X., Zhu Y. X., Mol. Cell Proteomics, 2010, 9(9), 2019
[13] Wang L., Li X. R., Lian H., Ni D. A., He Y. K., Chen X. Y., Ruan Y. L., Plant Physiol., 2010, 154(2), 744
[14] Ruan Y. L., Llewellyn D. J., Furbank R. T., The Plant Cell, 2003, 15(4), 952
[15] Scheibe R., Physiol. Plant, 2004, 120(1), 21
[16] Gietl C., Biochim. et Biophy. Acta, 1992, 1100(3), 217
[17] Backhausen J. E., Emmerlich A., Holtgrefe S., Horton P., Nast G.,Rogers J. J., Müller-Röber B., Scheibe R., Planta, 1998, 207(1), 105
[18] Wang Z. A., Li Q., Ge X. Y., Yang C. L., Luo X. L., Zhang A. H., Xiao J. L., Tian Y. C., Xia G. X., Chen X. Y., Sci. Rep., 2015, 5, 10343
[19] Ferguson D. L., Turley R. B., Triplett B. A., Meredith W. R., J. Agri. Food Chem., 1996, 44(12), 4022
[20] Ding Y., Ma Q. H., Biochimie, 2004, 86(8), 509
[21] Yao Y. X., Li M., Zhai H., You C. X., Hao Y. J., J. Plant Physiol., 2011, 168(5), 474
[22] Pines O., Shemesh S., Battat E., Goldberg I., Appl. Microbiol. Biotechnol., 1997, 48(2), 248
[23] Yao Y. X., Li M., Zhai H., You C. X., Hao Y. J., J. Plant Physiol., 2011, 168(5), 474
[24] Dhindsa R. S., Beasley C. A., Ting I. P., Plant Physiol., 1975, 56(3), 394
[25] Hagenbucher S., Olson D., Ruberson J., Wäckers F., Romeis J., Crit. Rev. Plant Sci., 2013, 32(6), 458
[26] Thompson J. D., Higgins D. G., Gibson T. J., Nucleic Acids Res., 1994, 22(22), 4673
[27] Tamura K., Stecher G., Peterson D., Filipski A., Kumar S., Mol. Biol. Evol., 2013, 30(12), 2725
[28] Han L. B., Li Y. B., Wang H. Y., Wu X. M., Li C. L., Luo M., Wu S. J., Kong Z. S., Pei Y., Jiao G. L., Xia G. X., The Plant Cell, 2013, 25(11), 4421
[29] Antoun M. D., Roberts M. F., Phytochemistry, 1975, 14(4), 909
[30] Timpa J. D., Burke J. J., Quisenberry J. E., Wendt C. W., Plant Physiol., 1986, 82(3), 724
[31] Pastore D., di Pede S., Passarella S., Plant Physiol., 2003, 133(4), 2029
[32] Imran M., Tang K., Liu J. Y., PLoS One, 2016, 11(11), e0166341
[33] Yao Y., Hao Y., Li M., Pang M., Liu Z., Zhai H., Front. Agric. China, 2008, 2(3), 307
[34] Lin C. F., Jiang R. H., Jiang L. Z., Qian X. Y., Attia K., Yang J. S., DNA Sequence, 2004, 15(4), 314
[35] Imran M., Liu J. Y., Pak. J. Bot., 2016, 48(3), 1081
[36] Miller S. S., Driscoll B. T., Gregerson R. G., Gantt J. S., Vance C. P., The Plant Journal, 1998, 15(2), 173
[37] Basara A. S., Malik C., J. Exp. Bot., 1983, 34(1), 1
[38] Li X. R., Wang L., Ruan Y. L., J. Exp. Bot., 2009, erp299
[39] Zhang B., Liu J. Y., Planta, 2016, 1
[40] Chen A., He S., Li F., Li Z., Ding M., Liu Q., Rong J., BMC Plant Biol., 2012, 12(1), 85
[41] Miller S. S., Driscoll B. T., Gregerson R. G., Gantt J. S., Vance C. P., The Plant Journal, 1998, 15(2), 173
[42] Cuevas I. C., Podestá F. E., Physiol. Plant., 2000, 108(3), 240
[43] Trípodi K. E., Podestá F. E., Plant Physiol. Biochem., 2003, 41(2), 97

Molecular Characterization of a Cytosolic Malate Dehydrogenase Gene(GhcMDH1) from Cotton

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