Effects of Protein on the Detection of Chlorobenzenes in Fish and Their Elimination by Modified QuEChERS Method

doi:10.1007/s40242-013-3230-4

Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (6): 1063-1067.doi: 10.1007/s40242-013-3230-4

• Articles • Previous Articles Next Articles

Effects of Protein on the Detection of Chlorobenzenes in Fish and Their Elimination by Modified QuEChERS Method

DENG Qiao-qiao, LI Gui-zhi, LIU Pei-pei, ZHANG Xing-mei, LIU Yong-ming

College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, P. R. China

Received:2013-05-21 Revised:2013-07-05 Online:2013-12-01 Published:2013-07-29
Contact: LIU Yong-ming E-mail:yongming_liu@163.com
Supported by:
Supported by Natural Science Foundation of Shandong Province, China(No.ZR2011BM009).

Abstract

Abstract:

Molecular fluorescence spectrometry, resonance Rayleigh scattering and gas chromatography-mass spectroscopy(GC-MS) were used to study the effect of pure fish serum albumin(FSA) as the model protein on the extraction of three chlorobenzenes(CBs: 1,3-DCB, 1,2-DCB and 1,2,4-TCB; DCB=dichlorobenzene; TCB=trichloroben-zene) in fish samples. The results show that there was a strong binding effect between CB and FSA. In an aqueous solution of 90%(volume fraction) acetone, a slow but full protein denaturation might take place, which would cause the unfolding of protein and the releasing of CBs. Based on these results, a QuEChERS(quick, easy, cheap, effective, rugged and safe) method was modified by replacing the traditional acetonitrile with acetone aqueous solution in the present work. This modified QuEChERS method was applied in the determination of CBs in fish samples. The spiked recoveries and the limits of detection were 80.4%―118.3% and 2.4―7.3 ng/g, respectively. This paper proposes a new strategy by slowing down the protein denaturation and releasing bound organic compounds to enhance the extraction efficiency of CBs in fish samples.

Key words: Chlorobenzene, Modified QuEChERS(quick, easy, cheap, effective, rugged and safe) method, Gas chromatography-mass spectroscopy, Fluorescence spectrometry, Resonance Rayleigh scattering

DENG Qiao-qiao, LI Gui-zhi, LIU Pei-pei, ZHANG Xing-mei, LIU Yong-ming. Effects of Protein on the Detection of Chlorobenzenes in Fish and Their Elimination by Modified QuEChERS Method[J]. Chemical Research in Chinese Universities, 2013, 29(6): 1063-1067.

References

[1] Kozani R. R., Assadi Y., Shemirani F., Hosseini M. R. M., Jamali M. R., Talanta, 2007, 72, 387
[2] Jia F. Y., Wang W. W., Wang J., Yin J. G., Liu Y. M., Liu Z. B., Anal. Methods, 2012, 4, 449
[3] Li G. Z., Liu Y. M., Guo X. Y., Wang J. J., Acta Chimica Sinica, 2006, 64, 679
[4] Wang X. R., Liu Z. B., Jia F. Y., Zhang Q., Yin J. G., Liu Y. M., Acta Chimica Sinica, 2011, 69, 2505
[5] Wang X. R., Zhu X. J., Liu Y. M., Chem. Res. Appl., 2010, 22, 763
[6] Wang Y., Lee H. K., J. Chromatogr. A, 1998, 803, 219
[7] Barro R., Ares S., Garcia-Jares C., Llompart M., Cela R., J. Chromatogr. A, 2004, 1045, 189
[8] Santos F., Sarrion M., Galceran M., J. Chromatogr. A, 1997, 771, 181
[9] Wennrich L., Popp P., Breuste J., Chromatographia Supplement, 2001, 53, 380
[10] Wells D. E., De Boer J., Tuinstra L. G. M. T., Reutergardh L., Griepink B., Fresenius Z Anal. Chem., 1988, 332, 591
[11] Oliver B. G., Nicol K. D., Chromatographia, 1982, 16, 336
[12] Zheng J. G., Zhou M. H., Li Z. J., Journal of Inspection and Quarantine, 2011, 21, 1
[13] van der Velde E. G., de Haan W., Liem A. K. D., J. Chromatogr. A, 1992, 626, 135
[14] Martínez E., Lacorte S., Llobet I., Viana P., Barceló D., J. Chromatogr. A, 2002, 959, 181
[15] Tao W. J., Huang Q., Li S. S., Rock and Mineral Analysis, 2009, 29, 543
[16] Bianchi F., Mattarozzi M., Betti P., Bisceglie F., Careri M., Mangia A., Sidisky L., Ongarato S., Dalcanale E., Anal. Chem., 2008, 80, 6423
[17] Tan G. H., Vijayaletchumy K., Pesticide Science, 1994, 40, 121
[18] Anastassiades M., Lehotay S. J., Stajnbaher D., Schenck F. J., J. AOAC Int., 2003, 86, 412
[19] Lehotay S. J., Kok A., Hiemstra M., Bodegraven P., J. AOAC Int., 2005, 88, 595
[20] Payá P., Anastassiades M., Mack D., Sigalova I., Tasdelen B., Oliva J., Barba A., Anal. Bioanal. Chem., 2007, 389, 1697
[21] Nguyen T. D., Yu J. E., Lee D. M., Lee G. H., Food Chem., 2008, 110, 207
[22] Rouvière F., Buleté A., Cren-Olivé C., Arnaudguilhem C., Talanta, 2012, 93, 336
[23] Jia F. Y., Wang W. W., Liu Z. B., Yin J. G., Liu Y. M., Anal. Chim. Acta, 2012, 70, 485
[24] Feng X. Z., Lin Z., Yang L. J., Wang C., Bai C. L., Talanta, 1998, 47, 1223
[25] Kandagal P. B., Ashoka S., Seetharamappa J., Shaikh S. M. T., Jadegoud Y., Ijare O. B., J. Pharm. Biomed. Anal., 2006, 41, 393
[26] Wang X. R., Han X. F., Jia F. Y., Wang R., Yin J. G., Liu Y. M., Journal of Yantai University(Natural Science and Engineering Edition), 2011, 24, 194
[27] Gao F. K., Chen L. S., Liu Y. M., Liu Z. B., Li G. Z., Anal. Chem., 2013, 41, 273
[28] Ross P. D., Subramanian S., Biochemistry, 1981, 20, 3096
[29] Vetri V., D'Amico M., Foderà V., Leone M., Ponzoni A., Sberveglieri G., Militello V., Arch. Biochem. Biophys., 2011, 508, 13
[30] Griebenow K., Klibanov A. M., J. Am. Chem. Soc., 1996, 118, 11695

Effects of Protein on the Detection of Chlorobenzenes in Fish and Their Elimination by Modified QuEChERS Method

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	SONG Yan-qi, LIU Shao-pu, LIU Zhong-fang and HU Xiao-li. Determination of Matrine and Oxymatrine in Radix Sophorae Flavescentis* by Resonance Rayleigh Scattering, Second-order Scattering and Frequency Doubling Scattering Technique [J]. Chemical Research in Chinese Universities, 2011, 27(5): 746-749.
[2]	HU Xiao-li*, LIU Sa, LIU Shao-pu, LIU Zhong-fang and SONG Yan-qi . Resonance Rayleigh Scattering and Resonance Nonlinear Scattering Methods for Determination of Methylene Blue with 12-Tungstophosphoric Acid [J]. Chemical Research in Chinese Universities, 2011, 27(2): 208-211.
[3]	HOU Ju-ying, AI Shi-yun* and SHI Wei-jie. Preparation and Characterization of Nano-Se/Silk Fibroin Colloids [J]. Chemical Research in Chinese Universities, 2011, 27(1): 158-160.
[4]	LIU Jian-wen*, LI Xin-hai, WANG Zhi-xing, GUO Hua-jun and HU Qi-yang. Preparation and Characterization of Phosphorus Pentafluoride Gas for Lithium Ion Battery Electrolyte Salt [J]. Chemical Research in Chinese Universities, 2009, 25(6): 791-795.
[5]	HU Rong, YANG Qiong, ZHANG Shu-ran and YANG Ji-dong*. Interaction Between Mitoxantrone Metal Complex and Titan Yellow by Resonance Rayleigh Scattering Spectra and Its Analytical Application [J]. Chemical Research in Chinese Universities, 2009, 25(3): 291-296.
[6]	YUE Bin, JIANG Lei, HU Chang-wen, CHEN Jian-min, HE He-yong. Heterogeneous Photocatalytic Mineralization of Chlorobenzene by Paratungstate-loaded Titania Catalysts in an Aqueous Medium [J]. Chemical Research in Chinese Universities, 2005, 21(4): 386-390.
[7]	Duan Yixiang, Kong Xiangxing, Zhang Hanqi, Liu Jun, Jin Qinhan . Evaluation of Atomic Fluorescence Spectrometry for Determination of Mercury Using a Low-Powered Argon Microwave Plasma Torch (MPT) [J]. Chemical Research in Chinese Universities, 1991, 7(1): 59-61.