Ultrasound-assisted Extraction of Kamebakaurin from Rabdosia excisa by Response Surface Methodology

doi:10.1007/s40242-013-3169-5

Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (6): 1072-1077.doi: 10.1007/s40242-013-3169-5

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Ultrasound-assisted Extraction of Kamebakaurin from Rabdosia excisa by Response Surface Methodology

TANG Jian¹, MA Rui-li¹, LI Qian-qian¹, JIANG Chun-bao¹, CHEN Rui¹, OUYANG Zhen¹, CHEN Hai-sheng², WANG Min³

1. School of Pharmacy, Jiangsu University, Zhenjiang 212013, P. R. China;
2. School of Pharmacy, Second Ministry Medical University, Shanghai 200433, P. R. China;
3. College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, P. R. China

Received:2013-04-12 Revised:2013-09-06 Online:2013-12-01 Published:2013-10-11
Contact: CHEN Hai-sheng E-mail:hschen2012@163.com
Supported by:
Supported by the National Natural Science Foundation of China(Nos.81072985, 21102057, 21272269), the Postdoctoral Foundation of Jiangsu Province, China(No.1201027B) and the China Postdoctoral Science Foundation(No.2012M521023).

Abstract

Abstract:

For the efficient extraction of kamebakaurin(KA), the ultrasound-assisted extraction(UAE) of KA from Rabdosia excisa(R. excisa) via response surface methodology(RSM) was investigated with high-performance liquid chromatography(HPLC). Effects of the experimental parameters such as extraction solvent, ratio of liquid to plant material, extraction time and extraction temperature on the extracting efficiency of KA from R. excisa were evaluated, and the purity of KA in residual was calculated. The optimized conditions were 65.5%(volume fraction) acetone, 35℃, time of 24.6 min with ultrasound of 80 W/L, 40 kHz, ratio of liquid to plant material at 30:1(mL/g). The maximum yield of KA is 0.708 mg/kg, with mean purity of 6.09%, indicating that ultrasound-assisted extraction is a feasible and useful method for extracting KA from R. excisa.

Key words: Ultrasound-assisted extraction, Kamebakaurin, Rabdosia excisa, Response surface methodology

TANG Jian, MA Rui-li, LI Qian-qian, JIANG Chun-bao, CHEN Rui, OUYANG Zhen, CHEN Hai-sheng, WANG Min. Ultrasound-assisted Extraction of Kamebakaurin from Rabdosia excisa by Response Surface Methodology[J]. Chemical Research in Chinese Universities, 2013, 29(6): 1072-1077.

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Ultrasound-assisted Extraction of Kamebakaurin from Rabdosia excisa by Response Surface Methodology

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