CRCU

Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (4): 667-670.doi: 10.1007/s40242-013-2352-z

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Forced Degradation of Flavonol Glycosides Extraced from Ginkgo biloba

JIN Ye1,3, ZHANG Wei-yu3, MENG Qing-fan1, LI Dan-hui2, GARG Sajay4, TENG Li-rong1, WEN Jing-yuan2   

  1. 1. College of Life Science, Jilin University, Changchun 130012, P. R. China;
    2. School of Pharmacy, Faculty of Medical and Health Sciences, University of Auckland, 1010 Auckland, New Zealand;
    3. School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, P. R. China;
    4. School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia 5001, Australia
  • Received:2012-09-12 Revised:2012-11-12 Online:2013-08-01 Published:2013-07-15
  • Contact: TENG Li-rong, WEN Jing-yuan E-mail:tenglirong@jlu.edu.cn;j.wen@auckland.ac.nz
  • Supported by:

    Supported by the Science and Technology Development Project of Jilin Province, China(No.20120935).

Abstract:

The degradation of flavonol glycosides extracted from Ginkgo biloba was performed under different conditions and the degraded products were determined by reversed-phase high performance liquid chromatography (RP-HPLC) method. Four stress conditions including acid(0.1 mol/L HCl), base(0.1 mol/L NaOH), temperature (70 ℃) and oxidation(0.03% H2O2, volume fraction) were used for the forced degradation studies. The pH stabilities of the flavonol glycosides were determined in phosphate buffers of varying pH values from 4.5 to 7.4. The degradation rate constants and half-life of three Ginkgo flavonol aglycones(quercetin, kaempferol and isorhamnetin) which represent Ginkgo flavonol glycosides were calculated in forced degradation and pH-stability studies of them. The results indicate that the three substances were more stable when incubated under acid condition and showed pH-dependent stability. The degradation was observed to follow first-order kinetics in all degradation studies. The stability results could provide important bases on development, preparation and storage of products of Ginkgo biloba extract and should be significantly considered during the further formulation development.

Key words: Ginkgo flavonol glycoside, Degradation, pH-Stability, Degradation rate constant