Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

doi:10.1007/s40242-013-2300-y

Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (2): 290-293.doi: 10.1007/s40242-013-2300-y

• Articles • Previous Articles Next Articles

Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

LI Wen-gui¹, DAI Fei-yan¹, CHENG Yong-xian², YIN Ge-fen¹, BI Jun-long¹, LI Dian-peng³

1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, P. R. China;
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, P. R. China;
3. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin 541006, P. R. China

Received:2012-08-13 Revised:2012-10-15 Online:2013-04-01 Published:2013-03-20
Supported by:
Supported by the Project of Talent Scholarship for the Youth of Yunnan Province of China(No.2007PY01-48), the Sino- German International Collaboration Project from Yunnan Province of China(No.2009AC011), the Project of Natural Compound Library Construction from Chinese Academy of Sciences(No.KSCX2-EW-R-15) and the Yunnan Provincial Program for Excellent Scientists, China(No.2009CI125).

Abstract

Abstract:

Porcine reproductive and respiratory syndrome(PRRS) caused by porcine reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious diseases in the swine industry worldwide, causing big economic losses. Vaccines are major weapons against PRRSV, however, current available vaccines have several limitations. Developing chemical drugs as alternatives is required. On the basis of traditional medical knowledge, we purposely selected 15 natural products originated from Chinese herbs with anti-infectious effects. Their antiviral activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 cells and reverse transcription polymerase chain reaction(RT-PCR) assay. Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits which could significantly reduce PRRSV-associated CPE with 50% inhibited concentration(IC₅₀) values of 7.9 and 39.4 mmol/L, respectively. Meanwhile, compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA expression of ORF7 gene in a dose-dependent manner. Study results suggest that compounds ethoxy- sanguinarine and atractylodinol may be useful anti-PRRSV drugs for swine industry or the hits for further lead optimization.

Key words: Porcine reproductive and respiratory syndrome virus(PRRSV), Natural product, Cytopathic effect, Antiviral activity

LI Wen-gui, DAI Fei-yan, CHENG Yong-xian, YIN Ge-fen, BI Jun-long, LI Dian-peng. Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products[J]. Chemical Research in Chinese Universities, 2013, 29(2): 290-293.

References

[1] Gorbalenya A. E., Enjuanes L., Ziebuhr J., Snijder E. J., Virus Res., 2006, 117(1), 17

[2] Polson D. D., Marsh W. E., Dial G. D.,North. Am. Food Anim. Pract., 1992, 8(3), 725

[3] Dee S. A., Joo H. S., Polson D. D., Marsh W. E., Vet. Rec., 1997, 140(19), 498

[4] Mengeling W. L., Lager K. M., Vorwald A. C., Am. J. Vet. Res., 1998, 59(12), 1540

[5] Meulenberg J. J., Vet. Res., 2000, 31(1), 11

[6] Yaeger M. J., Prieve T., Collins J., Christopher-Hennings J., Nelson E., Benfield D., J. Swine Health Prod., 1993, 1(5),7

[7] Wills R. W., Zimmerman J. J., Yoon K. J., Swenson S. L., Hoffman L. J., McGinley M. J., Hill H. T., Platt K. B., Vet. Microbiol., 1997, 57(1), 69

[8] Torremorell M., Pijoan C., Janni K., Walker R., Joo H. S., J. Vet. Res., 1997, 58(8), 828

[9] Huang Y. W., Meng X. J., Virus Res., 2010, 154(1/2), 141

[10] Thanawongnuwech R., Suradhat S., Virus Res., 2010, 154(1/2), 133

[11] Smith R. D., Soc. Sci. Med., 2006, 63(12), 3113

[12] Newman D. J., Cragg G. M., Snader K. M.,J. Nat. Prod., 2003, 66(7), 1022

[13] Duan B. F., Shen Y. P., Yang G. S., Zhang Y. F., Wu J. M., Duan G., Yin G. F., Prog. Vet. Med., 2010, 31, 11

[14] Tantular I. S., Kawamoto F., Trop. Med. Int. Health, 2003, 8(6), 569

[15] Ma S. C., He Z. D., Deng X. L., But P. P., Ooi V. E., Xu H. X., Lee S. H., Lee S. F., Chem. Pharm. Bull.(Tokyo), 2001, 49(11), 1471

[16] Pfaffl M. W., Nucleic Acids Res., 2001, 29(9), e45

[17] Kinghorn A. D., Pan L., Fletcher J. N., Chai H., J. Nat. Prod., 2011, 74(6), 1539

[18] Fabricant D. S., Farnsworth N. R., The Environ. Health Perspect., 2001, Suppl.1, 69

[19] Xue X., Zhang H. F., Bai X. H., Yue Y., Chem. J. Chinese Universities, 2012, 33(5), 942

[20] Zong M. R., Zhao Y. H., Zhang K., Yang L. F., Zheng Y. C., He C. Y., Chem. Res. Chinese Universities, 2011, 27(2), 241

[21] Kosina P., Walterova D., Ulrichova J., Lichnovsky V., Stiborova M., Rydlova H., Vicar J., Krecman V., Brabec M. J., Simanek V., Food Chem. Toxicol., 2004, 42(1), 85

[22] Luo G. F., Xiao S. J., Vet. Pharmaceut & Feed Addit., 2009, 14(5), 22

[23] Basini G., Santini S. E., Bussolati S., Grasselli F., J. Reprod. Dev., 2007, 53(3), 573

[24] Chan W. H., Toxicol. Lett., 2011, 205(3), 285

Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	LIU Dan, FAN Zi-chen, JIANG Jia-mei, WEI Jing, XIN Jian-chuang. Synthesis and Antiviral Activity of N-Adamantyl-2- amino(or 2-phenoxy)-acylamides [J]. Chemical Research in Chinese Universities, 2013, 29(4): 706-709.
[2]	JI Hai-tao, SHI Xiu-juan, HONG Yuan, WANG Ye, WANG Hui-ling, WANG Zhi-yong, .... Inhibition of Matrix Metalloproteinases Activities by Luteolin [J]. Chemical Research in Chinese Universities, 2009, 25(6): 895-898.
[3]	MA Jun-an, HUANG Run-qiu, FENG Lei, SONG Jian, QIU De-wen. Synthesis and Biological Activity of New Pyrethroid Acid Oxime-esters Containing Pyrazole Ring [J]. Chemical Research in Chinese Universities, 2003, 19(3): 297-301.