Multivalent Aptamer-modified DNA Origami as Drug Delivery System for Targeted Cancer Therapy

doi:10.1007/s40242-019-9273-4

高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (2): 254-260.doi: 10.1007/s40242-019-9273-4

Multivalent Aptamer-modified DNA Origami as Drug Delivery System for Targeted Cancer Therapy

CAO Mengyao¹, SUN Yueyang¹, XIAO Mingshu¹, LI Li¹, LIU Xiaohui², JIN Hong², PEI Hao¹

1. Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, P. R. China;
2. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, P. R. China

收稿日期:2019-10-10 修回日期:2019-10-24 出版日期:2020-04-01 发布日期:2019-10-29
通讯作者: PEI Hao E-mail:peihao@chem.ecnu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(No.21722502), the National Key R&D Program of China (No.2017YFC0906600), the National Key Research and Development Program of China for International Science & Innovation Cooperation Major Project between Governments(No.2018YFE0113200), the Shanghai Rising-star Program, China (No.19QA1403000) and the Foundation of the Shanghai Science and Technology Committee, China(No.18490740500).

Multivalent Aptamer-modified DNA Origami as Drug Delivery System for Targeted Cancer Therapy

CAO Mengyao¹, SUN Yueyang¹, XIAO Mingshu¹, LI Li¹, LIU Xiaohui², JIN Hong², PEI Hao¹

1. Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, P. R. China;
2. Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, P. R. China

Received:2019-10-10 Revised:2019-10-24 Online:2020-04-01 Published:2019-10-29
Contact: PEI Hao E-mail:peihao@chem.ecnu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21722502), the National Key R&D Program of China (No.2017YFC0906600), the National Key Research and Development Program of China for International Science & Innovation Cooperation Major Project between Governments(No.2018YFE0113200), the Shanghai Rising-star Program, China (No.19QA1403000) and the Foundation of the Shanghai Science and Technology Committee, China(No.18490740500).

摘要/Abstract

摘要： In spite of great development in nanoparticle-based drug delivery systems(DDSs) for improved therapeutic efficacy, it remains challenging for effective delivery of chemotherapeutic drugs to targeted tumor cells. In this work, we report a triangle DNA origami as targeted DDS for cancer therapy. DNA origami shows excellent biocompatibility and stability in cell culture medium for 24 h. In addition, the DNA origami structures conjugated with multivalent aptamers enable for efficient delivery of anticancer drug doxorubicin(Dox) into targeted cancer cell due to their targeting function, reducing side effects associated with nonspecific distribution. Moreover, we also demonstrated that the multivalent aptamer-modified DNA origami loading Dox exhibits prominent therapeutic efficacy in vitro. Accordingly, this work provides a good paradigm for the development of DNA origami nanostructure-based targeted DDS for cancer therapy.

关键词: DNA origami, Targeted drug delivery system, Aptamer

Abstract: In spite of great development in nanoparticle-based drug delivery systems(DDSs) for improved therapeutic efficacy, it remains challenging for effective delivery of chemotherapeutic drugs to targeted tumor cells. In this work, we report a triangle DNA origami as targeted DDS for cancer therapy. DNA origami shows excellent biocompatibility and stability in cell culture medium for 24 h. In addition, the DNA origami structures conjugated with multivalent aptamers enable for efficient delivery of anticancer drug doxorubicin(Dox) into targeted cancer cell due to their targeting function, reducing side effects associated with nonspecific distribution. Moreover, we also demonstrated that the multivalent aptamer-modified DNA origami loading Dox exhibits prominent therapeutic efficacy in vitro. Accordingly, this work provides a good paradigm for the development of DNA origami nanostructure-based targeted DDS for cancer therapy.

Key words: DNA origami, Targeted drug delivery system, Aptamer

CAO Mengyao, SUN Yueyang, XIAO Mingshu, LI Li, LIU Xiaohui, JIN Hong, PEI Hao. Multivalent Aptamer-modified DNA Origami as Drug Delivery System for Targeted Cancer Therapy[J]. 高等学校化学研究, 2020, 36(2): 254-260.

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Multivalent Aptamer-modified DNA Origami as Drug Delivery System for Targeted Cancer Therapy

Multivalent Aptamer-modified DNA Origami as Drug Delivery System for Targeted Cancer Therapy

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