Fluorescent Aptamer-Polyethylene Glycol Functionalized Graphene Oxide Biosensor for Profenofos Detection in Food

doi:10.1007/s40242-019-9257-4

高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (5): 787-794.doi: 10.1007/s40242-019-9257-4

Fluorescent Aptamer-Polyethylene Glycol Functionalized Graphene Oxide Biosensor for Profenofos Detection in Food

XIONG Jin'en, LI Shuang, LI Yi, CHEN Yingli, LIU Yu, GAN Junlan, JU Jiahui, XIAN Yaoling, XIONG Xiaohui

College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, P. R. China

收稿日期:2019-09-23 修回日期:2019-11-26 出版日期:2020-10-01 发布日期:2020-10-01
通讯作者: LI Yi, XIONG Xiaohui E-mail:liynj2012@njtech.edu.cn;xxh@njtech.edu.cn
基金资助:
Supported by the National Key R&D Program of China(No.2018YFC1602800), the Natural Science Foundation of Jiangsu Province, China(No.BK20161542) and the National Student's Platform for Innovation and Entrepreneurship Training Program, China(No.201910291084Z).

Fluorescent Aptamer-Polyethylene Glycol Functionalized Graphene Oxide Biosensor for Profenofos Detection in Food

XIONG Jin'en, LI Shuang, LI Yi, CHEN Yingli, LIU Yu, GAN Junlan, JU Jiahui, XIAN Yaoling, XIONG Xiaohui

College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, P. R. China

Received:2019-09-23 Revised:2019-11-26 Online:2020-10-01 Published:2020-10-01
Contact: LI Yi, XIONG Xiaohui E-mail:liynj2012@njtech.edu.cn;xxh@njtech.edu.cn
Supported by:
Supported by the National Key R&D Program of China(No.2018YFC1602800), the Natural Science Foundation of Jiangsu Province, China(No.BK20161542) and the National Student's Platform for Innovation and Entrepreneurship Training Program, China(No.201910291084Z).

摘要/Abstract

摘要： A biosensor based on self-assembled ssDNA(aptamer) and polyethylene glycol functionalized graphene oxide(GO-PEG) has been designed for sensing profenofos in food. The sensor has employed the fluorescence "on/off" switching strategy in a single step in homogeneous solution. Compared to traditional detection methods, the strategy proposed here is simple, convenient, fast and sensitive. Furthermore, compared with the general aptamer-GO structure, this aptamer-GO-PEG structure is in possession of a better detection performance, which is largely attributed to the improvement of the biocompatibility and the adjustment of the adsorption capacity of GO by grafting the blocking agent PEG onto the surface of GO. Additionally, the improved biocompatibility of GO shows better stability in salt solutions and physiological solutions, which is more conducive to its practical application in foods. In this project, profenofos had been detected with the proposed strategy, and the limit of detection has been controlled to be 0.21 ng/mL. This aptasensing assay has been applied to determining profenofos in (spiked)tap water, cabbage and milk with the recovery values ranging from 93.1% to 108.5%, from 90.8% to 113.2% and from 105.9% to 114.2%, respectively.

关键词: Aptamer, Graphene oxide, Polyethylene glycol, Profenofos

Abstract: A biosensor based on self-assembled ssDNA(aptamer) and polyethylene glycol functionalized graphene oxide(GO-PEG) has been designed for sensing profenofos in food. The sensor has employed the fluorescence "on/off" switching strategy in a single step in homogeneous solution. Compared to traditional detection methods, the strategy proposed here is simple, convenient, fast and sensitive. Furthermore, compared with the general aptamer-GO structure, this aptamer-GO-PEG structure is in possession of a better detection performance, which is largely attributed to the improvement of the biocompatibility and the adjustment of the adsorption capacity of GO by grafting the blocking agent PEG onto the surface of GO. Additionally, the improved biocompatibility of GO shows better stability in salt solutions and physiological solutions, which is more conducive to its practical application in foods. In this project, profenofos had been detected with the proposed strategy, and the limit of detection has been controlled to be 0.21 ng/mL. This aptasensing assay has been applied to determining profenofos in (spiked)tap water, cabbage and milk with the recovery values ranging from 93.1% to 108.5%, from 90.8% to 113.2% and from 105.9% to 114.2%, respectively.

Key words: Aptamer, Graphene oxide, Polyethylene glycol, Profenofos

XIONG Jin'en, LI Shuang, LI Yi, CHEN Yingli, LIU Yu, GAN Junlan, JU Jiahui, XIAN Yaoling, XIONG Xiaohui. Fluorescent Aptamer-Polyethylene Glycol Functionalized Graphene Oxide Biosensor for Profenofos Detection in Food[J]. 高等学校化学研究, 2020, 36(5): 787-794.

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Fluorescent Aptamer-Polyethylene Glycol Functionalized Graphene Oxide Biosensor for Profenofos Detection in Food

Fluorescent Aptamer-Polyethylene Glycol Functionalized Graphene Oxide Biosensor for Profenofos Detection in Food

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