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高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (1): 23-27.

• Articles • 上一篇    下一篇

Matrix Solid-phase Dispersion Extraction of Alkaloids from the Roots of Aconitum kusnezoffii Reichb.

WEI Wei, LI Xu-wen, SHI Xiao-lei, ZHOU Hong-yu, YANG Rui-jie, ZHANG Han-qi and JIN Yong-ri*   

  1. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • 收稿日期:2010-10-18 修回日期:2010-11-15 出版日期:2011-01-25 发布日期:2011-01-04
  • 通讯作者: JIN Yong-ri E-mail:jinyr@jlu.edu.cn
  • 基金资助:

    Supported by the Projects in the National Science & Technology Pillar Program During the Eleventh Five-Year Plan Period of China(No. 2006BAI14B01).

Matrix Solid-phase Dispersion Extraction of Alkaloids from the Roots of Aconitum kusnezoffii Reichb.

WEI Wei, LI Xu-wen, SHI Xiao-lei, ZHOU Hong-yu, YANG Rui-jie, ZHANG Han-qi and JIN Yong-ri*   

  1. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • Received:2010-10-18 Revised:2010-11-15 Online:2011-01-25 Published:2011-01-04
  • Contact: JIN Yong-ri E-mail:jinyr@jlu.edu.cn
  • Supported by:

    Supported by the Projects in the National Science & Technology Pillar Program During the Eleventh Five-Year Plan Period of China(No. 2006BAI14B01).

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摘要： Matrix solid-phase dispersion(MSPD) was developed for the extraction of four alkaloids, including aconitine, mesaconitine, hypaconitine and deoxyaconitine, from the roots of Aconitum kusnezoffii Reichb. The determination of the analyte was carried out by high performance liquid chromatography with UV detection. The alkaline alumina was used as sorbent. The mixture of acetonitrile and water was used as elution solvent. Several extraction parameters, such as type of sorbent, the ratio of sample to solid support material, type of the elution solvent and the volume of the elution solvent were tested. Mean recoveries ranged from 93.16% to 102.73%, with relative standard deviations from 0.27% to 4.17%. With the extraction efficiency and time expenditure taken into account, MSPD extraction should be a comparatively good method.

关键词: Matrix solid-phase dispersion, Aconitine-type alkaloid, Aconitum kusnezoffii Reichb., Extraction, High-performance liquid chromatography

Abstract: Matrix solid-phase dispersion(MSPD) was developed for the extraction of four alkaloids, including aconitine, mesaconitine, hypaconitine and deoxyaconitine, from the roots of Aconitum kusnezoffii Reichb. The determination of the analyte was carried out by high performance liquid chromatography with UV detection. The alkaline alumina was used as sorbent. The mixture of acetonitrile and water was used as elution solvent. Several extraction parameters, such as type of sorbent, the ratio of sample to solid support material, type of the elution solvent and the volume of the elution solvent were tested. Mean recoveries ranged from 93.16% to 102.73%, with relative standard deviations from 0.27% to 4.17%. With the extraction efficiency and time expenditure taken into account, MSPD extraction should be a comparatively good method.

Key words: Matrix solid-phase dispersion, Aconitine-type alkaloid, Aconitum kusnezoffii Reichb., Extraction, High-performance liquid chromatography