Simple and Sensitive Determination of Aromatic Acids in Coconut Water by g-C3N4@SiO2Based Solid-phase Extraction and HPLC-UV Analysis

doi:10.1007/s40242-018-8041-1

高等学校化学研究 ›› 2018, Vol. 34 ›› Issue (4): 528-535.doi: 10.1007/s40242-018-8041-1

Simple and Sensitive Determination of Aromatic Acids in Coconut Water by g-C₃N₄@SiO₂Based Solid-phase Extraction and HPLC-UV Analysis

QIANGBA Gezhen¹, ZHU Gangtian², YU Qiongwei¹, FENG Yuqi¹

1. Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China;
2. Key Laboratory of Tectonics and Petroleum Resources, Ministry of Education, China University of Geosciences, Wuhan 430075, P. R. China

收稿日期:2018-01-30 出版日期:2018-08-01 发布日期:2018-05-28
通讯作者: YU Qiongwei, FENG Yuqi E-mail:qwyu@whu.edu.cn;yqfeng@whu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(Nos.31671929, 21475098) and the Fundamental Research Funds for the Central Universities of China.

Simple and Sensitive Determination of Aromatic Acids in Coconut Water by g-C₃N₄@SiO₂Based Solid-phase Extraction and HPLC-UV Analysis

QIANGBA Gezhen¹, ZHU Gangtian², YU Qiongwei¹, FENG Yuqi¹

1. Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China;
2. Key Laboratory of Tectonics and Petroleum Resources, Ministry of Education, China University of Geosciences, Wuhan 430075, P. R. China

Received:2018-01-30 Online:2018-08-01 Published:2018-05-28
Contact: YU Qiongwei, FENG Yuqi E-mail:qwyu@whu.edu.cn;yqfeng@whu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.31671929, 21475098) and the Fundamental Research Funds for the Central Universities of China.

摘要/Abstract

摘要： In this study, graphitic carbon nitride deposited silica(g-C₃N₄@SiO₂) was prepared by simple pyrolysis of melamine on silica and then used as a solid-phase extraction(SPE) sorbent for the extraction of four representative aromatic acids including benzoic acid(BA), salicylic acid(SA), indolyl-3-butyricacid(IBA) and naphthalene acetic acid(NAA) in coconut water(CW) samples. g-C₃N₄@SiO₂ exhibited an excellent adsorption capacity for the four aromatic acids, which are in the concentration range of 500-558.8 μg/g. The four aromatic acids could be directly captured by g-C₃N₄@SiO₂ from CW samples within 5 min. Thus, a rapid, simple and effective method for the analysis of four aromatic acids in CW samples was developed by coupling g-C₃N₄@SiO₂-based SPE with high performance liquid chromatography-ultraviolet(HPLC-UV) detection. The linearity of the developed method was in the range of 20-1000 ng/mL and its limit of detection was in the range of 1.9-5.7 ng/mL, which were signi-ficantly lower than those of the reported similar methods. The intra-day and inter-day precisions(based on the relative standard deviation, n=3) of the four aromatic acids were under 9.5% and 10.4%, respectively. The developed method was applied to determining the four aromatic acids in real CW samples and the spiked recoveries varied from 81.1% to 121.8%.

关键词: Aromatic acid, Coconut water, Solid-phase extraction, Graphitic carbon nitride, High-performance liquid chromatography

Abstract: In this study, graphitic carbon nitride deposited silica(g-C₃N₄@SiO₂) was prepared by simple pyrolysis of melamine on silica and then used as a solid-phase extraction(SPE) sorbent for the extraction of four representative aromatic acids including benzoic acid(BA), salicylic acid(SA), indolyl-3-butyricacid(IBA) and naphthalene acetic acid(NAA) in coconut water(CW) samples. g-C₃N₄@SiO₂ exhibited an excellent adsorption capacity for the four aromatic acids, which are in the concentration range of 500-558.8 μg/g. The four aromatic acids could be directly captured by g-C₃N₄@SiO₂ from CW samples within 5 min. Thus, a rapid, simple and effective method for the analysis of four aromatic acids in CW samples was developed by coupling g-C₃N₄@SiO₂-based SPE with high performance liquid chromatography-ultraviolet(HPLC-UV) detection. The linearity of the developed method was in the range of 20-1000 ng/mL and its limit of detection was in the range of 1.9-5.7 ng/mL, which were signi-ficantly lower than those of the reported similar methods. The intra-day and inter-day precisions(based on the relative standard deviation, n=3) of the four aromatic acids were under 9.5% and 10.4%, respectively. The developed method was applied to determining the four aromatic acids in real CW samples and the spiked recoveries varied from 81.1% to 121.8%.

Key words: Aromatic acid, Coconut water, Solid-phase extraction, Graphitic carbon nitride, High-performance liquid chromatography

QIANGBA Gezhen, ZHU Gangtian, YU Qiongwei, FENG Yuqi. Simple and Sensitive Determination of Aromatic Acids in Coconut Water by g-C₃N₄@SiO₂Based Solid-phase Extraction and HPLC-UV Analysis[J]. 高等学校化学研究, 2018, 34(4): 528-535.

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Simple and Sensitive Determination of Aromatic Acids in Coconut Water by g-C₃N₄@SiO₂Based Solid-phase Extraction and HPLC-UV Analysis

Simple and Sensitive Determination of Aromatic Acids in Coconut Water by g-C₃N₄@SiO₂Based Solid-phase Extraction and HPLC-UV Analysis

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