CRCU

Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (5): 717-722.

• Articles • Previous Articles     Next Articles

Systemic Nuclear Proteomics Researches on Change of Jurkat T Lymphocyte Cells Under Radiation

LIANG Feng1, LIU Zhong-ying1, LIU Ning2, SONG Feng-rui2, ZHANG Xuan3* and LIU Zhi-qiang2*   

  1. 1. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
    2. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
    3. Research Center for Genomic Medicine and MH Radiobiology Research Unit, School of Public Health, Jilin University, Changchun 130021, P. R. China
  • Received:2010-01-21 Revised:2010-03-22 Online:2010-09-25 Published:2010-11-26
  • Contact: ZHANG Xuan. E-mail: zhangxuan2010@yahoo.com.cn; LIU Zhi-qiang. E-mail: liuzq@ciac.jl.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(Nos.20675079, 20873137 and 30672600).

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Abstract: Radiation causes severe constraint on numerous pathological functions of cells, such as cell growth,  nuclear genetic material expression and cell functions. In this study, we performed proteomic profiling of a nuclear fraction of Jurkat T lymphocyte cells under radiation along different time course by means of 2DE and MALDI TOF-MS. We found 24 protein spots whose expression had changed after radiation, including relevant proteins,  genetic material proteins, metabolism proteins, molecular chaperon and nuclear membrane proteins. Based on the above it is concluded that the combination of fluorescence labeled 2D-PAGE and MALDI-TOF MS is more precisely and effectively to elucidate the protein changes in Jurkat T lymphocyte cells after irradiation.

Key words: Nuclear proteome, Two-dimensional difference gel electrophoresis, MALDI-TOF-MS, Jurkat cell