Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (3): 420-426.

• Articles • Previous Articles     Next Articles

Expression of Human Interferon α 2b Gene in Ginseng Cells

REN Qi1,2, WANG Chun-yi3, SONG Zhi-ming4, LIU Dan2, YU Hai-peng2 and SHENG Jun1,5*   

  1. 1. College of Life Science, Jilin University, Changchun 130012, P. R. China;
    2. Changchun Institute of Biological Products, Changchun 130062, P. R. China;
    3. Liaoning Yisheng Biopharmaceutical Co., Ltd., Shengyang 110131, P. R. China;
    4. First Affiliated Hospital, Jilin University, Changchun 130031, P. R. China;
    5. Yunnan Agricultural University, Kunming 650201, P. R. China
  • Received:2009-06-23 Revised:2009-10-14 Online:2010-05-25 Published:2010-07-27
  • Contact: SHENG Jun. E-mail: shengjun@ccidp.com
  • Supported by:

    Supported by the Science and Technology Development Planning Foundation of Jilin Province of China(No.20030405).

Abstract:

The human interferon α 2b(hIFN-α2b) gene was cloned into binary vector pBI121 to obtain plant expression vector pBIFN. The recombinant plasmid pBIFN was transferred into Agrobacterium tumefaciens strain LBA4404. Then the hIFN-α2b gene was introduced into ginseng callus cells via Agrobacterium-mediated transformation and the ginseng cell line carrying hIFN-α2b gene was selected on G418-containing medium. The presence of target gene in transformed cells was confirmed by PCR and RT-PCR. The results indicate that hIFN-α2b gene has been integrated into the ginseng cells’ genome, with transcription products. hIFN-α2b expressed by the transgenic ginseng cells was detected by Western blot. It was shown that a specific protein band at 19000 could be observed. Cytopathic effect(CPE) inhibition assay using the WISH-VSV system shows that the mean antiviral activity of expressed hIFN-α2b was 6.0×104 IU/mL.

Key words: Human interferon α 2b; Ginseng cell; Plant transformation; Agrobacterium tumefaciens