Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (3): 402-405.

• Articles • Previous Articles     Next Articles

Synthesis and Spectral Characterization of Novel Sulfhydryl-reactive Benzimidazole Fluorescent Probes

LEI Ying-jie*, OUYANG Jie*, ZHANG You-lai and DING Mei   

  1. Department of Chemistry & Chemical Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China
  • Received:2009-03-24 Revised:2009-09-08 Online:2010-05-25 Published:2010-07-27
  • Contact: LEI Ying-jie. E-mail: lyj@tjut.edu.cn; OUYANG Jie. E-mail: ouyang@tjut.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20771082), the Natural Science Foundation of    Tianjin Municipality, China(No.09JCYBJC05800) and the Educational Commission of Tianjin Municipality, China (No.20080509).

Abstract:

Three sulfhydryl-reactive fluorescent probes, which contain 2-(1H-benzoimidazol-2-yl)-phenol and maleimide group, were synthesized and their structures were characterized by IR and 1H NMR. Their photo-physical properties and the specificity to sulfhydryl group in the 2-mercapto-ethanol as well as the L-cysteine adducts were indicated by fluorescence intensity. Preliminary tests revealed that the probes could react selectively with the cysteine molecules, suggesting that these probes might be generally useful in biomedical researches.

Key words: Fluorescent probe; 2-(2-Hydroxyphenyl) benzimidazole; Sulfhydryl-reactive