Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (3): 366-370.

• Articles • Previous Articles     Next Articles

Resonance Light Scattering Method for Determination of Amikacin with Potassium Ferrioxalate as a Probe

HU Xiao-li*, AN Lan-xiang, LIU Shao-pu, LIU Zhong-fang and LI Cui-xia   

  1. Key Laboratory of Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China
  • Received:2009-06-15 Revised:2009-07-19 Online:2010-05-25 Published:2010-07-27
  • Contact: HU Xiao-li. E-mail: xiaolihu@swu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20875078) and Chongqing Municipal Key Laboratory on Luminescence and Real-Time Analysis, Southwest University, China(No.2006CA8006).

Abstract:

In a weak acid medium, potassium ferrioxalate(PF) can react with some aminoglycoside(AGs) antibiotics, such as amikacin(AMK), kanamycin(KANA), tobramycin(TOB) and gentamicin(GEN), to form ion-association complexes. It results in the enhancement of resonance light scattering(RLS) in different degrees. The maximum scattering peaks are all located at 345 nm. Among them, the relative scattering intensity(ΔIRLS) of AMK system is much higher than that of KANA, TOB or GEN. Therefore the method is more propitious to the determination of trace amounts of AMK. The optimum reaction conditions, influencing factors, and the relationship between scattering intensity and concentration of antibiotics were investigated by means of the proposed method. The enhancement of RLS signals is directly proportional to the concentration of antibiotics in a certain range of concentration. A new resonance light scattering method for the determination of AMK and other aminoglycoside antibiotics with [Fe(C2O4)3]3– as a probe is thus established based on it. The method exhibits high sensitivity and good selectivity. The detection limit(3σ) for AMK is 1.8 ng/mL. The method can be applied to the determination of AMK in clinical serum samples. The reaction mechanism and the reasons for RLS enhancement are discussed in this paper.

Key words: Resonance light scattering; Amikacin; Aminoglycoside antibiotics; Potassium ferrioxalate