Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (1): 110-113.

• Articles • Previous Articles     Next Articles

Catalytic Characteristics of Lipase Immobilized in CTAB/TX-100 MBG

SONG Shao-fang1* and LUAN Yu-xia2   

  1. 1. College of Chemistry and Material Science, Shandong Agricultural University, Tai’an 271018, P. R. China;
    2. Institute of Pharmaceutics, School of Pharmaceutical Science, Shandong University, Jinan 250100, P. R. China
  • Received:2009-02-25 Revised:2009-03-24 Online:2010-01-04 Published:2010-03-29
  • Contact: SONG Shao-fang. E-mail: ssf@sdau.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No. 20803044) and the Foundation of Key Laboratory of Colloid and Interface Chemistry of Ministry of Education, Shandong University, China(No.200714).

Abstract:

CTAB/TX-100 microemulsion-based gel(MBG), which could be soaked in aqueous solution for a long time without mechanical strength and shape changes, was successfully prepared. Lipase immobilized in this gel had a higher activity than that in CTAB MBG when catalyzed the esterification reaction between n-hexanoic acid and n-octanol and its enantioselectivity was slightly higher when catalyzed the stereoselective esterification reaction between racemic ibuprofen and n-octanol. In aqueous solution, lipase in CTAB/TX-100 MBG could catalyze smoothly the hydrolysis reaction of olive oil and could be reused many times without the evident change of MBG mechanical strength although its activity decreased 41.8% after nine-time recycles.

Key words: Microemulsion-based gel(MBG); Immobilized lipase; Activity; Stereoselectivity; Esterification; Hydrolysis