Protein@AuNPs Synthesized by Microfluidic Droplet System and Application in Cu(II) and L-Cysteine Sensing

doi:10.1007/s40242-025-4203-0

Chemical Research in Chinese Universities ›› 2025, Vol. 41 ›› Issue (1): 131-137.doi: 10.1007/s40242-025-4203-0

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Protein@AuNPs Synthesized by Microfluidic Droplet System and Application in Cu(II) and L-Cysteine Sensing

ZHU Xiaotong¹, LI Xinyu¹, QI Li², ZHANG Rongyue¹, LI Nan¹, HE Xiaonan¹, QIAO Juan¹

1. College of New Materials and Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, P. R. China;
2. Beijing National Laboratory for Molecular Sciences, Key Lab of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China

Received:2024-10-07 Online:2025-02-01 Published:2025-01-18
Contact: QIAO Juan,qiaojuan@bipt.edu.cn E-mail:qiaojuan@bipt.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (No. 22074148), the Beijing Municipal Natural Science Foundation, China (No. 2242005), the Zhiyuan Science Foundation of Beijing Institute of Petrochemical Technology (BIPT), China (No. 2024004), and the Fund of the Beijing Institute of Petrochemical Technology Analytical Testing Research Center, China.

Abstract

Abstract: In this study, Pepsin@AuNPs (Pep@AuNPs) and Trypsin@AuNPs (Try@AuNPs) were synthesized by a microfluidic droplet system using Pepsin and Trypsin as protection reagents and NaOH as reducing reagents. Compared to the synthesis method in a flask, the AuNPs synthesized by the microfluidic droplet system demonstrated uniform nucleation, superior ultraviolet absorption performance, high stability and short preparation cycles (15 min). The detection range of Cu(II) by Pep@AuNPs was 1.0—100.0 μmol/L and the detection limit was 0.3 μmol/L. The detection range of L-Cysteine by Try@AuNPs was 0.3—250.0 mmol/L and the detection limit was 0.1 mmol/L. This universal method provides an effective strategy for the detection of bioactive molecules, such as metal ions and amino acids by AuNPs with protein as a protective agent.

Key words: Microfluidic droplet system, AuNPs, Trypsin, Pepsin

ZHU Xiaotong, LI Xinyu, QI Li, ZHANG Rongyue, LI Nan, HE Xiaonan, QIAO Juan. Protein@AuNPs Synthesized by Microfluidic Droplet System and Application in Cu(II) and L-Cysteine Sensing[J]. Chemical Research in Chinese Universities, 2025, 41(1): 131-137.

References

[1] Soderling T. R., J. Biol. Chem., 1976, 251, 4359.
[2] Aggarwal S., Ikram S., Int. J. Biol. Macromol., 2022, 207, 205.
[3] Hinterwirth H., Lindner W., Lämmerhofer M., Anal. Chim. Acta, 2012, 733, 90.
[4] Nidhin M., Ghosh D., Yadav H., Yadav N., Majumber S., Mater. Sci. Eng. B, 2015, 202, 46.
[5] Gole A., Dash C., Rao M., Sastry M., Chem. Commun., 2000, 4, 297.
[6] Zhang F., Li Y., Jafari S. M., Liu Y., Sang Y., Wang S., Wang X., Food Chem., 2024, 450, 139311.
[7] Caracciolo G., Farokhzad O. C., Mahmoudi M., Trends Biotechnol., 2017, 35, 257.
[8] Li X. G., Guo W., Xu R. A., Song Z. Z., Ni T. T., Spectrochim. Acta A, 2022, 272, 120983.
[9] Wang G. K., Liu X. B., Yan C. L., Bai G. Y., Lu Y., Colloid Surface B, 2015, 135, 261.
[10] Boleininger J., Kurz A., Reuss V., Sönnichsen C., Phys. Chem. Chem. Phys., 2006, 8, 3824.
[11] Lazarus L. L., Yang A. S. J., Chu S., Brutchey R. L., Malmstadt N., Lab on a Chip, 2010, 10, 3377.
[12] Lin X. Z., Terepka A. D., Yang H., Nano Lett., 2004, 4, 2227.
[13] Jeong G. Y., Ricco R., Liang K., Ludwig J., Kim J. O., Falcaro P., Kim D. P., Chem. Mater., 2015, 27, 7903.
[14] Chen T. Y., Yin S., Wu J., Trac-Trend Anal. Chem., 2021, 142, 116309.
[15] Seaberg J., Kaabipour S., Hemmati S., Ramsey J. D., Eur. J. Pharm. Biopharm., 2020, 154, 127.
[16] Lee T. Y., Choi T. M., Shim T. S., Frijns R. A., Kim S. H., Lab on a Chip, 2016, 16, 3415.
[17] Qiao J., Ding H., Liu Q., Zhang R. Y., Qi L., Anal. Chem., 2017, 89, 2080.
[18] Dalibera N. C., Rodrigues-Jesus M. J., Andreata-Santos R., Janini L. M. R., Oliveira A. F., Rodrigues A. A., Favaro M. T., ACS Appl. Nano Mater., 2023, 6, 22774.
[19] Shrimal P., Jadeja G., Patel S., Chem. Eng. Res. Des., 2020, 153, 728.
[20] Ma Q. M., Cao J., Gao Y., Han S. C., Liang Y., Zhang T. T., Wang X. Y., Sun Y., Nanoscale, 2020, 12, 15512.
[21] Chen Y., Zhao D., Xiao F., Li X. Y., Su Z. W., Jiang X. Y., Adv. Mater., 2023, 35, 2209672.
[22] Haghighinia A., Movahedirad S., J. Flow Chem., 2022, 12, 337.
[23] Shepherd S. J., Issadore D., Mitchell M. J., Biomaterials, 2021, 274, 120826.
[24] Ohta R., Morikawa K., Tsuyama Y., Kitamori T., J. Micromech. Microeng., 2023, 34, 017002.
[25] Gao S. Y., Xu T. G., Wu L., Zhu X. Y., Wang X. F., Chen Y., Li G., Li X. X., Biosensors, 2024, 14, 114.
[26] Zhang J., Xu W. H., Xu F. Y., Lu W. W., Hu L. Y., Zhou J. L., Zhang C., Jiang Z., J. Food Eng., 2021, 290, 110212.
[27] Bai X., Yu X. A., Zhang R., Zhang Y., Hu Y., Zhao L., Zhang M., Tian J., Yu B. Y., ACS Appl. Nano Mater., 2021, 4, 4919.
[28] Mishra A., Das P. K., Phys. Chem. Chem. Phys., 2022, 24, 22464.
[29] Correira J. M., Handali P. R., Webb L. J., J. Chem. Phys., 2022, 157, 0909002.
[30] Dridi N., Jin Z., Perng W., ACS Nano, 2024, 18, 8649.
[31] Caracciolo G., Farokhzad O. C., Mahmoudi M., Trends Biotechnol., 2017, 35, 257.
[32] Salelles L., Floury J., Le Feunteun S., Food Funct., 2021, 12, 12468.
[33] Rio A. R. D., Keppler J. K., Boom R. M., Janssen A. E., Food Funct., 2021, 12, 4570.
[34] Santos M. P., Ferreira M. A., Junior E. C., Bonomo R. C., Veloso C. M., Bioproc. Biosyst. Eng., 2023, 46, 1651.
[35] Sannaikar M. S., Inamdar L. S., Inamdar S. R., J. Mol. Liq., 2019, 281, 156.
[36] Wang G., Wang W. L., Shangguan E., Gao S. Y., Liu Y. F., Mater. Sci. Eng. C, 2020, 111, 110830.
[37] Nidhin M., Ghosh D., Yadav H., Yadav N., Majumder S., Mater. Sci. Eng. B, 2015, 202, 46.
[38] Wang G., Liu X. B., Yan C. L., Bai G. Y., Lu Y., Colloids Surfaces B, 2015, 135, 261.
[39] Hinterwirth H., Lindner W., Lämmerhofer M., Anal. Chim. Acta, 2012, 733, 90.
[40] Yadavali S., Jeong H. H., Lee D., Issadore D., Nat. Commun., 2018, 9, 1222.
[41] Paul I. E., Rajeshwari A., Prathna T. C., Raichur A. M., Chandrasekaran N., Mukherjee A., Anal. Methods, 2015, 7, 1453.
[42] Elfiky A. A., Ibrahim I. M., Elghareib A. M., Bashandy Y. S., Samir A., Hamdy M. M., Kamal R. T., Amin F. G., Elkaramany Y., Rashad A. M., Abdelaziz Y. S., Fathey M. M., Comput. Biol. Med., 2023, 164, 107363.
[43] Luo Y., Miao H., Yang X., Talanta, 2015, 144, 488.

Protein@AuNPs Synthesized by Microfluidic Droplet System and Application in Cu(II) and L-Cysteine Sensing

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