HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana

doi:10.1007/s40242-015-4184-5

Chemical Research in Chinese Universities ›› 2015, Vol. 31 ›› Issue (1): 38-43.doi: 10.1007/s40242-015-4184-5

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HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana

WANG Hui, WANG Jiayuan, WU Xiaopeng, SHEN Haiyan, LUO Ying, DAI Haofu, MEI Wenli

Hainan Key Laboratory for Research and Development of Natural Products from Li Folk Medicine, Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, P. R. China

Received:2014-05-14 Revised:2014-09-26 Online:2015-02-01 Published:2014-10-13
Contact: DAI Haofu, E-mail:daihaofu@itbb.org.cn;MEI Wenli, E-mail:meiwenli@itbb.org.cn E-mail:daihaofu@itbb.org.cn;meiwenli@itbb.org.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.31070321), the National Support Science and Techno-logy Subject of China(No.2013BAI11B04), the Fundamental Research Funds for Central Public Welfare Research Institutes, Chinese Academy of Tropical Agricultural Sciences(No.1630052012014) and the Major Technology Project of Hainan Province, China(No. ZDZX2013008-4).

Abstract

Abstract:

Dragon's blood is a traditional medicine and used in many countries with different cultures because of its various therapeutic properties. The main bioactive constituents of dragon's blood are flavonoids, which exhibit va-rious pharmaceutical activities, such as haemostatic, analgesic, anticoagulant, and antimicrobial activities and so on, and have attracted the attention of researchers on the development of new drugs. However, the formation process of dragon's blood in nature is very slow, which cannot meet the demand of pharmaceutical uses. Dracaena cambodiana (D. cambodiana) is one of the resource plants of dragon's blood. During the course of tissue culture of D. cambodiana, 6-benzylaminopurine was added in the medium, then red secretion was discovered in the cultured medium. Twelve compounds(1―12), including 11 flavonoids, were determined via the analysis of constituents of the cultured medium by high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS) and the results were compared with that of the standards isolated from dragon's blood. Among compounds 1―12, 4,4'-dihydroxy-2-methoxydihydrochalcone(5), 4,4'-dihydroxy-2,6-dimethoxydihydrochalcone(6) and (2S)-7,3'-dihydroxy-4'-methoxyflavane(9) were major compounds of the red secretion, with contents of 15.70, 7.10 and 57.23 mg/L, respectively. Therefore, it is promising that flavonoids from dragon's blood can be obtained from the tissue culture of its resource plants for the purpose of drug development.

Key words: Flavonoid, Dragon's blood, High performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-ESI-MS), Dracaena cambodiana, Tissue culture

WANG Hui, WANG Jiayuan, WU Xiaopeng, SHEN Haiyan, LUO Ying, DAI Haofu, MEI Wenli. HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana[J]. Chemical Research in Chinese Universities, 2015, 31(1): 38-43.

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HPLC-ESI-MS Analysis of Flavonoids Obtained from Tissue Culture of Dracaena cambodiana

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