Abstract: Three wavelength spectrophotometry was used to determine the content ofbenoxinate hydrochloride.Using this method could effectively eliminate the devia-tion of background absorption caused by the change of concentration and the errorof quantitative anaiysis caused by asymmetric peaks, and at the same time the lean-ing degree of base line was corrected.This method was simple, the recovery was 98.62% 101.86% and the coefficient of variation was 0.551%.

Key words: Spectrophotometry, Three wavelength, Benoxinate hydrochloride