Abstract: Three polysaccharides, TAA, TAB, TAChave been isolated from the pollen of Typha angustifolia L., and purified by hot water extraction, alcohol precipitation, Sevage method, gel filtration (Sephadex G50) and ion-exchange chromatography on DEAE Sephadex A25 and DEAE-cellulose. Their purities were tested by gel filtration and electrophoresis. Their structures were characterized by paper chromatography of the hydrolysis products, GLCanalysis of the alditol acetates, alkylation analysis, ¹H NMRand ¹³C NMRspectra. TAAis mainly composed of a-L-arabinofuanose, β-D-galactose and α-D-galacturonic acid, while the backbones of TABand TACare composed of (1→5)-linked α- L-arabinofuranosyl residues.

Key words: Typha angustifolia L., pollen polysaccharide, alkylation analysis