CRCU

Chemical Research in Chinese Universities ›› 2006, Vol. 22 ›› Issue (1): 29-32.

• Articles • Previous Articles     Next Articles

Effects of Nitrobenzenes on DNA Damage in Germ Cells of Rats

XU Jing-bo1, JING Ti-song2, YANG Li1, SUN Zhi-wei3, SHI Long3   

  1. 1. Department of Environmental Science, Northeast Normal University, Changchun 130024, P. R. China;
    2. College of Environment and Resource, Jilin University, Changchun 130026, P. R. China;
    3. Public Health College, Jilin University, Changchun 130021, P. R. China
  • Received:2005-03-02 Online:2006-02-24 Published:2011-08-06
  • Supported by:

    Supported by National Natural Science Foundation of China(No.30370262), Natural Science Foundation of Jilin Province(No.20020633-2), National Basic Research Program(973 Project) of China(No.2004CB418505) and Subject Construction of Doctoral Degree by the Ministry of Education of China(No.20040200002).

PDF (PC)

Cited

52

Abstract: The single cell gel electrophoresis(SCGE) technique was used to detect DNA damage in germ cells of rats, which was induced by five tested nitroaromatic compounds. Mixed cultures of Sertoli and germ cells were prepared from the rats testis and their responses to m-dinitrobenzene(m-DNB), 2,4-dinitrotoluene(2,4-DNT), 2,6-dinitroto-luene(2,6-DNT), 4-nitrotoluene(4-NT) and 2,4-dinitroaniline(2,4-DNAn) were studied. The results show that all the five chemicals have a reproductive toxicity. Each dose group and the control group were significantly different(P<0.01). All of them can lead to the damage to DNA in the germ cells of Kunming male rats in the definite range of concentration(m-DNB: 0.04-25 μmol/L; 2,4-DNT, 2,6-DNT and 4-NT: 0.032-500 μmol/L; 2,4-DNAn: 0.8-20 μmol/L). When the concentration increases, the damage rate will become higher, which shows an evident logarithm dose-effect relationship.

Key words: Nitroaromatic compound, Sertoli-germ cell coculture, SCGE, DNA damage