CRCU

Chemical Research in Chinese Universities ›› 2004, Vol. 20 ›› Issue (6): 707-710.

• Articles • Previous Articles     Next Articles

Isolation and Extraction of Total Flavonoids from Epimedium Koreanum Nakai by Supercritical Fluid Extraction

LIU Chun-ming1,2, ZHAO Xue-liang3, LIU Zhi-qiang1, XING Jun-peng1   

  1. 1. Laboratory of New Drug Research, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China;
    2. Department of Chemistry, Changchun Teachers College, Changchun 130032, P. R. China;
    3. China-Japan Union Hospital, Jilin University, Changchun 130032, P. R. China
  • Received:2004-01-10 Online:2004-12-24 Published:2011-08-06
  • Supported by:

    Supported by the Natural Science and Technology Foundation of Jilin Province(No.20020637-1).

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Abstract: In the present paper is reported the method for the isolation and extraction of total flavonoids of Epimedium Koreanum Nakai by means of supercritical fluid extraction(SFE). By examining pressure, temperature, amounts of modifier and extraction time, the optimized condition of SFE is confirmed as 30 MPa and 60 ℃, with 70% ethanol as the modifier. The samples were statically extracted for 30 min, followed by dynamic extraction for 120 min at a flow rate of 6 mL/min. The quantitative analysis of total flavonoids was performed by UV-Vis spectrophotometry. Compared with the conventional method, the SFE method is more efficient, more rapid and more friendly environmentally.

Key words: Supercritical fluid extraction(SFE), Flavonoids, Epimedium Koreanum Naka