Chemical Research in Chinese Universities ›› 2005, Vol. 21 ›› Issue (2): 196-200.

• Articles • Previous Articles     Next Articles

Effect of Nano-anatase TiO2 on Spectral Characterization of Photosystem Ⅱ Particles from Spinach

HONG Fa-shui1, YANG Ping2, GAO Feng-qing1, LIU Chao1, ZHENG Lei1, ZHOU Juan1   

  1. 1. College of Life Sciences;
    2. College of Chemistry and Chemical Engineering, Suzhou University, Suzhou 215006, P. R. China
  • Received:2004-06-21 Online:2005-03-24 Published:2011-07-27
  • Supported by:

    Supported by the Natural Science Foundation of Jiangsu Province Universities of China(No.03KJB180122).

Abstract: The photosystem I (PS Ⅱ) particles were purified by means of nano-anatase TiO2 treatment of spinach and studied by spectroscopy.The results show that the electron transport and the oxygen-evolving rate of PS I are accelerated after it has been treated with nano-anatase TiO2; the UV-Vis absorption spectrum of PS I particles is increased; the red shift of fluorescence emission peak of PS I is 2 nm; the peak intensity is decreased; the PS Ⅱ signal I s of low temperature electron paramagnetic resonanace(EPR) spectrum is intensified under light, and the PS I circular dichroism(CD) spectrum is similar to that of control.It is suggested that nano-anatase TiO2 might bind to the PS I reaction center complex and intensify the function of the PS I electron donor, however, nano-anatase TiO2 treatment does not change the configuration of the PS Ⅱ reaction center complex.

Key words: Nano-anatase TiO2, Spinach, Photosystem Ⅱ, Spectral characterization