Aptamers as Recognition Elements for Electrochemical Detection of Exosomes

doi:10.1007/s40242-022-2088-8

高等学校化学研究 ›› 2022, Vol. 38 ›› Issue (4): 879-885.doi: 10.1007/s40242-022-2088-8

Aptamers as Recognition Elements for Electrochemical Detection of Exosomes

CHANG Kaili, SUN Peng, DONG Xin, ZHU Chunnan, LIU Xiaojun, ZHENG Dongyun, LIU Chao

Key Laboratory of Cognitive Science of State Ethnic Affairs Commission, Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis and Treatment, College of Biomedical Engineering, South-Central Minzu University, Wuhan 430074, P. R. China

收稿日期:2022-03-11 修回日期:2022-04-11 出版日期:2022-08-01 发布日期:2022-07-01
通讯作者: ZHENG Dongyun;LIU Chao E-mail:dongyun1203@163.com;chaoliu@scuec.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (Nos.22004134, 21804146) and the Fundamental Research Funds for the Central Universities, South-Central Minzu University, China(Nos.CZY20006, CZQ21025).

Aptamers as Recognition Elements for Electrochemical Detection of Exosomes

CHANG Kaili, SUN Peng, DONG Xin, ZHU Chunnan, LIU Xiaojun, ZHENG Dongyun, LIU Chao

Key Laboratory of Cognitive Science of State Ethnic Affairs Commission, Hubei Key Laboratory of Medical Information Analysis and Tumor Diagnosis and Treatment, College of Biomedical Engineering, South-Central Minzu University, Wuhan 430074, P. R. China

Received:2022-03-11 Revised:2022-04-11 Online:2022-08-01 Published:2022-07-01
Contact: ZHENG Dongyun;LIU Chao E-mail:dongyun1203@163.com;chaoliu@scuec.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos.22004134, 21804146) and the Fundamental Research Funds for the Central Universities, South-Central Minzu University, China(Nos.CZY20006, CZQ21025).

摘要/Abstract

摘要： Exosome analysis is emerging as an attractive noninvasive approach for disease diagnosis and treatment monitoring in the field of liquid biopsy. Aptamer is considered as a promising molecular probe for exosomes detection because of the high binding affinity, remarkable specificity, and low cost. Recently, many approaches have been developed to further improve the performance of electrochemical aptamer based(E-AB) sensors with a lower limit of detection. In this review, we focus on the development of using aptamer as a specific recognition element for exosomes detection in electrochemical sensors. We first introduce recent advances in evolving aptamers against exosomes. Then, we review methods of immobilization aptamers on electrode surfaces, followed by a summary of the main strategies of signal amplification. Finally, we present the insights of the challenges and future directions of E-AB sensors for exosomes analysis.

关键词: Aptamer, Exosome, Biosensor, Diagnosis, Biomolecular recognition

Abstract: Exosome analysis is emerging as an attractive noninvasive approach for disease diagnosis and treatment monitoring in the field of liquid biopsy. Aptamer is considered as a promising molecular probe for exosomes detection because of the high binding affinity, remarkable specificity, and low cost. Recently, many approaches have been developed to further improve the performance of electrochemical aptamer based(E-AB) sensors with a lower limit of detection. In this review, we focus on the development of using aptamer as a specific recognition element for exosomes detection in electrochemical sensors. We first introduce recent advances in evolving aptamers against exosomes. Then, we review methods of immobilization aptamers on electrode surfaces, followed by a summary of the main strategies of signal amplification. Finally, we present the insights of the challenges and future directions of E-AB sensors for exosomes analysis.

Key words: Aptamer, Exosome, Biosensor, Diagnosis, Biomolecular recognition

CHANG Kaili, SUN Peng, DONG Xin, ZHU Chunnan, LIU Xiaojun, ZHENG Dongyun, LIU Chao. Aptamers as Recognition Elements for Electrochemical Detection of Exosomes[J]. 高等学校化学研究, 2022, 38(4): 879-885.

CHANG Kaili, SUN Peng, DONG Xin, ZHU Chunnan, LIU Xiaojun, ZHENG Dongyun, LIU Chao. Aptamers as Recognition Elements for Electrochemical Detection of Exosomes[J]. Chemical Research in Chinese Universities, 2022, 38(4): 879-885.

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Aptamers as Recognition Elements for Electrochemical Detection of Exosomes

Aptamers as Recognition Elements for Electrochemical Detection of Exosomes

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