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高等学校化学研究 ›› 2002, Vol. 18 ›› Issue (1): 12-15.

• Articles • 上一篇    下一篇

A Chemiluminescence Optical Fiber Glucose Biosensor Based on Co-immobilizing Glucose Oxidase and Horseradish Peroxidase in a Sol-gel Film

LI Ying-xiu1, ZHU Lian-de1, ZHU Guo-yi1, ZHAO Chun2   

  1. 1. State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
    2. College of Chemistry, Jilin University, Changchun 130023, P. R. China
  • 收稿日期:2000-12-05 出版日期:2002-01-24 发布日期:2011-08-04

A Chemiluminescence Optical Fiber Glucose Biosensor Based on Co-immobilizing Glucose Oxidase and Horseradish Peroxidase in a Sol-gel Film

LI Ying-xiu1, ZHU Lian-de1, ZHU Guo-yi1, ZHAO Chun2   

  1. 1. State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
    2. College of Chemistry, Jilin University, Changchun 130023, P. R. China
  • Received:2000-12-05 Online:2002-01-24 Published:2011-08-04

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摘要： An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0.2-2 mmol/L and the detection limit was approximately 0.12 mmol/L. The relative standard deviation was 5.3% (n=6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.

关键词: Fiber optical biosensor, Sol-gel, Chemiluminescence, Glucose oxidase, Horseradish peroxidase, Glucose assay

Abstract: An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0.2-2 mmol/L and the detection limit was approximately 0.12 mmol/L. The relative standard deviation was 5.3% (n=6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.

Key words: Fiber optical biosensor, Sol-gel, Chemiluminescence, Glucose oxidase, Horseradish peroxidase, Glucose assay