Facile Route to Enzyme Immobilization: Gloucose Oxidase Entrapped in Titania Under Mild Environmental Conditions and Consequent Electrochemical Sensor Response

doi:10.1007/s40242-018-7231-1

高等学校化学研究 ›› 2018, Vol. 34 ›› Issue (3): 490-494.doi: 10.1007/s40242-018-7231-1

Facile Route to Enzyme Immobilization: Gloucose Oxidase Entrapped in Titania Under Mild Environmental Conditions and Consequent Electrochemical Sensor Response

YU Zhihui¹, LIU Dandan¹, LI Yunqiao²

1. Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, P. R. China;
2. National Institute of Metrology, Beijing 100029, P. R. China

收稿日期:2017-07-03 修回日期:2017-11-03 出版日期:2018-06-01 发布日期:2017-12-04
通讯作者: YU Zhihui E-mail:yuzhihui@bjut.edu.cn
基金资助:
Supported by the General Program of Science and Technology Development Project of Beijing Municipal Education Commission of China(No.KM201210005008), the National Natural Science Foundation of China(No.21275014) and the Program for New Century Excellent Talents in University, China(No.NCET-12-0603).

Facile Route to Enzyme Immobilization: Gloucose Oxidase Entrapped in Titania Under Mild Environmental Conditions and Consequent Electrochemical Sensor Response

YU Zhihui¹, LIU Dandan¹, LI Yunqiao²

1. Key Laboratory of Beijing on Regional Air Pollution Control, College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, P. R. China;
2. National Institute of Metrology, Beijing 100029, P. R. China

Received:2017-07-03 Revised:2017-11-03 Online:2018-06-01 Published:2017-12-04
Contact: YU Zhihui E-mail:yuzhihui@bjut.edu.cn
Supported by:
Supported by the General Program of Science and Technology Development Project of Beijing Municipal Education Commission of China(No.KM201210005008), the National Natural Science Foundation of China(No.21275014) and the Program for New Century Excellent Talents in University, China(No.NCET-12-0603).

摘要/Abstract

摘要： A facile one-step method to the immobilization of the combination of glucose oxidase(GOD) and catalase(CAT) in mesostructured TiO₂ was proposed. The results obtained by transmission electron microspectroscopy and nitrogen adsorption-desorption analysis clearly show that the TiO₂ mediated by the combination of GOD and CAT(CGC) has a large surface area and a narrow pore-size distribution. The CGC immobilized on mesostructured TiO₂ exhibits direct electrochemistry and good electrocatalytic performance without any electron mediator.

关键词: Direct electrochemistry, Glucose oxidase, Immobilization

Abstract: A facile one-step method to the immobilization of the combination of glucose oxidase(GOD) and catalase(CAT) in mesostructured TiO₂ was proposed. The results obtained by transmission electron microspectroscopy and nitrogen adsorption-desorption analysis clearly show that the TiO₂ mediated by the combination of GOD and CAT(CGC) has a large surface area and a narrow pore-size distribution. The CGC immobilized on mesostructured TiO₂ exhibits direct electrochemistry and good electrocatalytic performance without any electron mediator.

Key words: Direct electrochemistry, Glucose oxidase, Immobilization

YU Zhihui, LIU Dandan, LI Yunqiao. Facile Route to Enzyme Immobilization: Gloucose Oxidase Entrapped in Titania Under Mild Environmental Conditions and Consequent Electrochemical Sensor Response[J]. 高等学校化学研究, 2018, 34(3): 490-494.

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Facile Route to Enzyme Immobilization: Gloucose Oxidase Entrapped in Titania Under Mild Environmental Conditions and Consequent Electrochemical Sensor Response

Facile Route to Enzyme Immobilization: Gloucose Oxidase Entrapped in Titania Under Mild Environmental Conditions and Consequent Electrochemical Sensor Response

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