Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A

doi:10.1007/s40242-019-8322-3

高等学校化学研究 ›› 2019, Vol. 35 ›› Issue (3): 382-389.doi: 10.1007/s40242-019-8322-3

Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A

WANG Chengke^1,2, TAN Rong¹, LI Jiangyu¹, ZHANG Zexiang¹

1. College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P. R. China;
2. Henan Key Laboratory of Biomolecular Recognition and Sensing, Shangqiu Normal University, Shangqiu 476000, P. R. China

收稿日期:2018-10-03 修回日期:2019-01-03 出版日期:2019-06-01 发布日期:2019-02-25
通讯作者: WANG Chengke E-mail:wangck@ujs.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(No.21305032), the China Postdoctoral Science Foundation (No.2014M551522), the Postdoctoral Science Foundation of Jiangsu Province, China(No.1402073B), the Henan Key Laboratory of Biomolecular Recognition and Sensing(Shangqiu Normal University), China(No.HKLBRSK1803) and the Hong Kong Scholar Program, China (No.XJ2017008).

Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A

WANG Chengke^1,2, TAN Rong¹, LI Jiangyu¹, ZHANG Zexiang¹

1. College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P. R. China;
2. Henan Key Laboratory of Biomolecular Recognition and Sensing, Shangqiu Normal University, Shangqiu 476000, P. R. China

Received:2018-10-03 Revised:2019-01-03 Online:2019-06-01 Published:2019-02-25
Contact: WANG Chengke E-mail:wangck@ujs.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21305032), the China Postdoctoral Science Foundation (No.2014M551522), the Postdoctoral Science Foundation of Jiangsu Province, China(No.1402073B), the Henan Key Laboratory of Biomolecular Recognition and Sensing(Shangqiu Normal University), China(No.HKLBRSK1803) and the Hong Kong Scholar Program, China (No.XJ2017008).

摘要/Abstract

摘要： A double magnetic separation-assisted fluorescence method was developed to rapidly detect ochratoxin A(OTA). The OTA aptamer functionalized magnetic nanomaterial(Fe₃O₄-Aptamer) and complementary DNA conjugated nitrogen-doped graphene quantum dots(NGQDs-cDNA) were used in this assay. Aptamer could hybridize with cDNA, which induced the NGQDs-cDNA to bind onto Fe₃O₄-Aptamer, and resulted in the fluorescence quenching of NGQDs. After the addition of OTA, the NGQDs-cDNA could release into the solution, and resulted in the recovery of fluorescence signal of NGQDs consequently. By utilizing the magnetic separation, the unbonded NGQDs-cDNA and residual Fe₃O₄-Aptamer were removed, which significantly increased the fluorescence signal intensity. OTA could be detected in the linear range of 10 nmol/L to 2000 nmol/L, with a limit of detection as 0.66 nmol/L. The advantages of this method include simple operation, good selectivity and high sensitivity, and this method can be used for the rapid detection of ochratoxin A in wheat and corn.

关键词: Ochratoxin A, Fe₃O₄, Magnetic separation, Fluorescence method, Aptamer

Abstract: A double magnetic separation-assisted fluorescence method was developed to rapidly detect ochratoxin A(OTA). The OTA aptamer functionalized magnetic nanomaterial(Fe₃O₄-Aptamer) and complementary DNA conjugated nitrogen-doped graphene quantum dots(NGQDs-cDNA) were used in this assay. Aptamer could hybridize with cDNA, which induced the NGQDs-cDNA to bind onto Fe₃O₄-Aptamer, and resulted in the fluorescence quenching of NGQDs. After the addition of OTA, the NGQDs-cDNA could release into the solution, and resulted in the recovery of fluorescence signal of NGQDs consequently. By utilizing the magnetic separation, the unbonded NGQDs-cDNA and residual Fe₃O₄-Aptamer were removed, which significantly increased the fluorescence signal intensity. OTA could be detected in the linear range of 10 nmol/L to 2000 nmol/L, with a limit of detection as 0.66 nmol/L. The advantages of this method include simple operation, good selectivity and high sensitivity, and this method can be used for the rapid detection of ochratoxin A in wheat and corn.

Key words: Ochratoxin A, Fe₃O₄, Magnetic separation, Fluorescence method, Aptamer

WANG Chengke, TAN Rong, LI Jiangyu, ZHANG Zexiang. Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A[J]. 高等学校化学研究, 2019, 35(3): 382-389.

WANG Chengke, TAN Rong, LI Jiangyu, ZHANG Zexiang. Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A[J]. Chemical Research in Chinese Universities, 2019, 35(3): 382-389.

参考文献 48

[1]	Al-Anati L., Petzinger E., J. Vet. Pharmacol. Ther., 2006, 29, 79
[2]	Meulenberg E. P., Toxins(Basel), 2012, 4, 244
[3]	Wu X., Hu J., Zhu B., Lu L., Huang X., Pang D., J. Chromatogr. A, 2011, 1218, 7341
[4]	Bazin I., Nabais E., Lopez-Ferber M., Toxins(Basel), 2010, 2, 2230
[5]	Pfohl-Leszkowicz A., Manderville R. A., Mol. Nutr. Food. Res., 2007, 51, 61
[6]	Novo P., Moulas G., Prazeres D. M. F., Chu V., Conde J. P., Sensors Actuators B:Chem., 2013, 176, 232
[7]	Jo E. J., Mun H., Kim S. J., Shim W. B., Kim M. G., Food Chem., 2016, 194, 1102
[8]	Pittet A., Royer D., J. Agric. Food Chem., 2002, 50, 243
[9]	Skarkova J., Ostry V., Malir F., Roubal T., Anal. Lett., 2013, 46, 1495
[10]	Zhou J., Xu J. J., Huang B. F., Cai Z. X., Ren Y. P., J. Sep. Sci., 2017, 40, 2141
[11]	Mo J., Gong Q., Zhou H., Bai X., Tan J., Peng Z., Huang Y., Journal of Food Safety and Quality, 2016, 7, 182
[12]	Andrade M. A., Lanças F. M., J. Chromatogr. A, 2017, 1493, 41
[13]	Bougrini M., Baraket A., Jamshaid T., El Aissari A., Bausells J., Zabala M., El Bari N., Bouchikhi B., Jaffrezic-Renault N., Abdelhamid E., Sensors Actuators B:Chem., 2016, 234, 446
[14]	Karczmarczyk A., Baeumner A. J., Feller K. H., Electrochimica Acta, 2017, 243, 82
[15]	Zhou J., Rossi J., Nature Reviews Drug Discovery, 2017, 16, 181
[16]	Hamaguchi N., Ellington A., Stanton M., Anal. Biochem., 2001, 294, 126
[17]	Kolpashchikov D. M., J. Am. Chem. Soc., 2005, 127, 12442
[18]	Bai H. Y., Del Campo F. J., Tsai Y. C., Biosens. Bioelectron., 2013, 42, 17
[19]	Wang Q. Y., Kang Y. J., Nanoscale Research Letters, 2016, 11, 150
[20]	Wandtke T., Woniak J., Kopiński P., Viruses, 2015, 7, 751
[21]	Farokhzad O. C., Jon S., Khademhosseini A., Tran T. N. T., LaVan D. A., Langer R., Cancer Res., 2004, 64, 7668
[22]	Shim W. B., Mun H., Joung H. A., Ofori J. A., Chung D. H., Kim M. G., Food Control, 2014, 36, 30
[23]	Wang C. K., Tan R., Chen D., Talanta, 2018, 182, 363
[24]	Goud K. Y., Hayat A., Satyanarayana M., Kumar V. S., Catanante G., Gobi K. V., Marty J. L., Microchimica Acta, 2017, 184, 4401
[25]	Lim S. Y., Shen W., Gao Z., Chem. Soc. Rev., 2015, 44, 362
[26]	Dey S., Govindaraj A., Biswas K., Rao C., Chem. Phys. Lett., 2014, 595, 203
[27]	Ju J., Chen W., Anal. Chem., 2015, 87, 1903
[28]	Tian Y., Wang F., Liu Y., Pang F., Zhang X., Electrochimica Acta, 2014, 146, 646
[29]	Thanh T. D., Balamurugan J., Lee S. H., Kim N. H., Lee J. H., Biosens. Bioelectron., 2016, 81, 259
[30]	Anuar N. S., Basirun W. J., Ladan M., Shalauddin M., Mehmood M. S., Sensors Actuators B:Chem., 2018, 266, 375
[31]	Ju J., Chen W., Biosens. Bioelectron., 2014, 58, 219
[32]	Cai F., Liu X., Liu S., Liu H., Huang Y., RSC Advances, 2014, 4, 52016
[33]	Akin I., Arslan G., Tor A., Ersoz M., Cengeloglu Y., J. Hazard. Mater., 2012, 235, 62
[34]	Xu S., Zhao Y., Zheng F., Zhang Y., Journal of Materials Science, 2016, 51, 2550
[35]	Sun L., Li Y., Sun M., Wang H., Xu S., Zhang C., Yang Q., New J. Chem., 2011, 35, 2697
[36]	Tamer U., Gündodu Y., Boyac. H., Pekmez K., J. Nanopart. Res., 2010, 12, 1187
[37]	Yang X., Zhang X., Ma Y., Huang Y., Wang Y., Chen Y., J. Mater. Chem., 2009, 19, 2710
[38]	Kohler N., Sun C., Fichtenholtz A., Gunn J., Fang C., Zhang M., Small, 2006, 2, 785
[39]	Wang S., Zhang Y., Pang G., Zhang Y., Guo S., Anal. Chem., 2017, 89, 1704
[40]	Zhao Y., Li J., Zhao L., Zhang S., Huang Y., Wu X., Wang X., Chem. Eng. J., 2014, 235, 275
[41]	Wang C., Qian J., Wang K., Yang X., Liu Q., Hao N., Wang C., Dong X., Huang X., Biosens. Bioelectron., 2016, 77, 1183
[42]	Li Y., Sun L., Qian J., Wang C., Liu Q., Han E., Hao N., Zhang L., Cai J., Wang K., Anal. Chim. Acta, 2016, 948, 90
[43]	Wang A. J., Li Y. F., Li Z. H., Feng J. J., Sun Y. L., Chen J. R., Materials Science and Engineering:C, 2012, 32, 1640
[44]	Zhu X., Zuo X., Hu R., Xiao X., Liang Y., Nan J., Mater. Chem. Phys., 2014, 147, 963
[45]	Castillo G., Lamberti I., Mosiello L., Hianik T., Electroanalysis, 2012, 24, 512
[46]	Wang C., Dong X., Liu Q., Wang K., Anal. Chim. Acta, 2015, 860, 83
[47]	Al-Taher F., Cappozzo J., Zweigenbaum J., Lee H. J., Jackson L., Ryu D., Food Control, 2017, 72, 27
[48]	Soler M., Belushkin A., Cavallini A., Kebbi-Beghdadi C., Greub G., Altug H., Biosens. Bioelectron., 2017, 94, 560

Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A

Double Magnetic Separation-assisted Fluorescence Method for Sensitive Detection of Ochratoxin A

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