Mechanisms of Cytochrome C Extraction by Reverse Micelles

高等学校化学研究 ›› 2001, Vol. 17 ›› Issue (1): 73-76.

Mechanisms of Cytochrome C Extraction by Reverse Micelles

YU Yan-chun¹, QIAN Bao-hua¹, CHU Ying², WU Zi-sheng², GAO Chang-qing²

1. Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, P. R. China;
2. Department of Chemistry, Northeast Normal University, Changchun 130024, P. R. China

收稿日期:2000-03-01 出版日期:2001-01-24 发布日期:2011-08-04

Mechanisms of Cytochrome C Extraction by Reverse Micelles

YU Yan-chun¹, QIAN Bao-hua¹, CHU Ying², WU Zi-sheng², GAO Chang-qing²

1. Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005, P. R. China;
2. Department of Chemistry, Northeast Normal University, Changchun 130024, P. R. China

Received:2000-03-01 Online:2001-01-24 Published:2011-08-04

摘要/Abstract

摘要： The extraction of cytochrome C was carried out by means of phase transfer technique with three different reverse micellar systems, i.e., a CTAB micellar solution in n-butyl alcohol-chloroform(volume ratio 4∶1), an AOT micellar solution in isooctane and a SDSS-D₂EHPA micellar solution in isooctane. The extraction mechanisms were studied. The results show that the extraction mechanisms for the same proteins with different types of reverse micellar systems can be distinct. The extraction of cytochrome C with CTAB and SDSS-D₂EHPA reverse micellar systems are carried out according to the mechanism of electrostatic interaction. However, in the extraction of cytochrome C with the AOT reverse micellar system, the electrostatic interaction between the protein and the surfactant is not important.

关键词: Mechanism, Cytochrome C, Extraction, Reverse micelles

Abstract: The extraction of cytochrome C was carried out by means of phase transfer technique with three different reverse micellar systems, i.e., a CTAB micellar solution in n-butyl alcohol-chloroform(volume ratio 4∶1), an AOT micellar solution in isooctane and a SDSS-D₂EHPA micellar solution in isooctane. The extraction mechanisms were studied. The results show that the extraction mechanisms for the same proteins with different types of reverse micellar systems can be distinct. The extraction of cytochrome C with CTAB and SDSS-D₂EHPA reverse micellar systems are carried out according to the mechanism of electrostatic interaction. However, in the extraction of cytochrome C with the AOT reverse micellar system, the electrostatic interaction between the protein and the surfactant is not important.

Key words: Mechanism, Cytochrome C, Extraction, Reverse micelles

YU Yan-chun, QIAN Bao-hua, CHU Ying, WU Zi-sheng, GAO Chang-qing. Mechanisms of Cytochrome C Extraction by Reverse Micelles[J]. 高等学校化学研究, 2001, 17(1): 73-76.

YU Yan-chun, QIAN Bao-hua, CHU Ying, WU Zi-sheng, GAO Chang-qing. Mechanisms of Cytochrome C Extraction by Reverse Micelles[J]. Chemical Research in Chinese Universities, 2001, 17(1): 73-76.

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Mechanisms of Cytochrome C Extraction by Reverse Micelles

Mechanisms of Cytochrome C Extraction by Reverse Micelles

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