Enzyme-driven Nanorobots Walking Along Predesigned Tracks on the DNA Origami for Cargo Transport and Catalysis

doi:10.1007/s40242-024-4025-5

高等学校化学研究 ›› 2024, Vol. 40 ›› Issue (2): 333-342.doi: 10.1007/s40242-024-4025-5

• Articles • 上一篇

Enzyme-driven Nanorobots Walking Along Predesigned Tracks on the DNA Origami for Cargo Transport and Catalysis

NIE Cunpeng, MA Tianran, CHEN Tingting, CHU Xia

State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China

收稿日期:2024-01-29 修回日期:2024-03-04 出版日期:2024-04-01 发布日期:2024-03-27
通讯作者: CHU Xia xiachu@hnu.edu.cn;CHEN Tingting chenting1104@hnu.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (Nos. 21991080, 22374042), and the Science and Technology Major Project of Hunan Province, China (No. 2021SK1020).

Enzyme-driven Nanorobots Walking Along Predesigned Tracks on the DNA Origami for Cargo Transport and Catalysis

NIE Cunpeng, MA Tianran, CHEN Tingting, CHU Xia

State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China

Received:2024-01-29 Revised:2024-03-04 Online:2024-04-01 Published:2024-03-27
Contact: CHU Xia xiachu@hnu.edu.cn;CHEN Tingting chenting1104@hnu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (Nos. 21991080, 22374042), and the Science and Technology Major Project of Hunan Province, China (No. 2021SK1020).

摘要/Abstract

摘要： In molecular engineering, designing and synthesizing molecular machines with capable of performing complex tasks, remains a formidable challenge. DNA is an excellent candidate for building molecular robots because it is highly programmable. Here, we present an artificial nanorobot, in which a DNA cube serves as the inert ‘body’, and nucleic acid catalysts based on an enzymatic nicking reaction act as the ‘legs’ for walking. The nanorobot can execute a series of actions, such as ‘start’, ‘turn’, and ‘stop’ when it walks along a predefined track. Its performance could be confirmed and monitored by using an atomic force microscope (AFM) and fluorescence spectroscopy. Inspired by biological machines, we artificially designed a series of specialized tasks that combined walking with control of cargo transport and catalysis. Real-time fluorescence kinetics curves provide monitoring signals for cargo transport and catalytic processes. Our work can enrich the toolbox of DNA machinery and has great potential for engineering molecular nanofactories.

关键词: DNA origami, DNA nanorobot, Controlled cargo transport, DNAzyme catalysis

Abstract: In molecular engineering, designing and synthesizing molecular machines with capable of performing complex tasks, remains a formidable challenge. DNA is an excellent candidate for building molecular robots because it is highly programmable. Here, we present an artificial nanorobot, in which a DNA cube serves as the inert ‘body’, and nucleic acid catalysts based on an enzymatic nicking reaction act as the ‘legs’ for walking. The nanorobot can execute a series of actions, such as ‘start’, ‘turn’, and ‘stop’ when it walks along a predefined track. Its performance could be confirmed and monitored by using an atomic force microscope (AFM) and fluorescence spectroscopy. Inspired by biological machines, we artificially designed a series of specialized tasks that combined walking with control of cargo transport and catalysis. Real-time fluorescence kinetics curves provide monitoring signals for cargo transport and catalytic processes. Our work can enrich the toolbox of DNA machinery and has great potential for engineering molecular nanofactories.

Key words: DNA origami, DNA nanorobot, Controlled cargo transport, DNAzyme catalysis

NIE Cunpeng, MA Tianran, CHEN Tingting, CHU Xia. Enzyme-driven Nanorobots Walking Along Predesigned Tracks on the DNA Origami for Cargo Transport and Catalysis[J]. 高等学校化学研究, 2024, 40(2): 333-342.

NIE Cunpeng, MA Tianran, CHEN Tingting, CHU Xia. Enzyme-driven Nanorobots Walking Along Predesigned Tracks on the DNA Origami for Cargo Transport and Catalysis[J]. Chemical Research in Chinese Universities, 2024, 40(2): 333-342.

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Enzyme-driven Nanorobots Walking Along Predesigned Tracks on the DNA Origami for Cargo Transport and Catalysis

Enzyme-driven Nanorobots Walking Along Predesigned Tracks on the DNA Origami for Cargo Transport and Catalysis

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