A Triode-like Enzyme-free Catalytic Circuit with Junction Fuel

doi:10.1007/s40242-019-0025-2

高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (2): 261-267.doi: 10.1007/s40242-019-0025-2

A Triode-like Enzyme-free Catalytic Circuit with Junction Fuel

LUO Tao, WANG Xiaojing, FAN Sisi, LIU Yan, CHENG Jin, TANG Linlin, SONG Jie

Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China

收稿日期:2019-10-28 修回日期:2019-11-19 出版日期:2020-04-01 发布日期:2019-11-20
通讯作者: SONG Jie E-mail:sjie@sjtu.edu.cn
基金资助:
Supported by the National Key Research and Development Program of China(No.2017YFC1200904), the National Natural Science Foundation of China(Nos.11761141006, 21605102) and the Natural Science Foundation of Shanghai, China (Nos.19520714100, 19ZR1475800).

A Triode-like Enzyme-free Catalytic Circuit with Junction Fuel

LUO Tao, WANG Xiaojing, FAN Sisi, LIU Yan, CHENG Jin, TANG Linlin, SONG Jie

Institute of Nano Biomedicine and Engineering, Department of Instrument Science and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, P. R. China

Received:2019-10-28 Revised:2019-11-19 Online:2020-04-01 Published:2019-11-20
Contact: SONG Jie E-mail:sjie@sjtu.edu.cn
Supported by:
Supported by the National Key Research and Development Program of China(No.2017YFC1200904), the National Natural Science Foundation of China(Nos.11761141006, 21605102) and the Natural Science Foundation of Shanghai, China (Nos.19520714100, 19ZR1475800).

摘要/Abstract

摘要： Target detection circuits have been previously designed, which are propelled by conventional PCR, isothermal amplification and strand-displacement reaction. These detection circuits obtain the target signal via the replication of the target strand, the aggregation of the signal particles or the branch migration. Here we constructed a triode-like enzyme-free catalyst strand-displacement circuit for target DNA detection. The target strand triggered the reaction and released the fluorescence signal strand circularly through branch migration. However, the main challenge of strand-displacement reaction is the signal leakage. Therefore, we designed a double strand structure "junction fuel", which was used to increase the binding energy across the displacement process. Ultimately, the leakage of the system obtained stable inhabitation due to the junction fuel strand. The limit of detection of the system was as low as 0.11 nmol/L and the gain of the system was as high as 28-fold(the concentration of target was 50 nmol/L). Furthermore, the process of the system was visualized vividly in the reaction curve through the kinetic simulation implemented, which suggests that the combination of the kinetic simulation and the experiment exhibits a promising prospect towards the use of strand-displacement circuit in analytical, diagnostic application and synthetic biology.

关键词: Catalysis, Strand-displacement, Junction fuel, Triode-like, Kinetic simulation

Abstract: Target detection circuits have been previously designed, which are propelled by conventional PCR, isothermal amplification and strand-displacement reaction. These detection circuits obtain the target signal via the replication of the target strand, the aggregation of the signal particles or the branch migration. Here we constructed a triode-like enzyme-free catalyst strand-displacement circuit for target DNA detection. The target strand triggered the reaction and released the fluorescence signal strand circularly through branch migration. However, the main challenge of strand-displacement reaction is the signal leakage. Therefore, we designed a double strand structure "junction fuel", which was used to increase the binding energy across the displacement process. Ultimately, the leakage of the system obtained stable inhabitation due to the junction fuel strand. The limit of detection of the system was as low as 0.11 nmol/L and the gain of the system was as high as 28-fold(the concentration of target was 50 nmol/L). Furthermore, the process of the system was visualized vividly in the reaction curve through the kinetic simulation implemented, which suggests that the combination of the kinetic simulation and the experiment exhibits a promising prospect towards the use of strand-displacement circuit in analytical, diagnostic application and synthetic biology.

Key words: Catalysis, Strand-displacement, Junction fuel, Triode-like, Kinetic simulation

LUO Tao, WANG Xiaojing, FAN Sisi, LIU Yan, CHENG Jin, TANG Linlin, SONG Jie. A Triode-like Enzyme-free Catalytic Circuit with Junction Fuel[J]. 高等学校化学研究, 2020, 36(2): 261-267.

LUO Tao, WANG Xiaojing, FAN Sisi, LIU Yan, CHENG Jin, TANG Linlin, SONG Jie. A Triode-like Enzyme-free Catalytic Circuit with Junction Fuel[J]. Chemical Research in Chinese Universities, 2020, 36(2): 261-267.

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A Triode-like Enzyme-free Catalytic Circuit with Junction Fuel

A Triode-like Enzyme-free Catalytic Circuit with Junction Fuel

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