Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement

doi:10.1007/s40242-018-8185-z

高等学校化学研究 ›› 2018, Vol. 34 ›› Issue (6): 952-958.doi: 10.1007/s40242-018-8185-z

Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement

ZHENG Jingyi, FENG Rongrong, HE Caimei and LI Xiaoxia

School of Chemistry and Chemical Engineering, Yan'an University, Yan'an 716000, P. R. China

收稿日期:2018-06-04 出版日期:2018-12-01 发布日期:2018-09-29
通讯作者: LI Xiaoxia E-mail:ydlixiaoxia@yau.edu.cn
基金资助:
Supported by the Natural Science Foundation of Yan'an University, China(No.YDY2018-07).

Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement

ZHENG Jingyi, FENG Rongrong, HE Caimei and LI Xiaoxia

School of Chemistry and Chemical Engineering, Yan'an University, Yan'an 716000, P. R. China

Received:2018-06-04 Online:2018-12-01 Published:2018-09-29
Contact: 10.1007/s40242-018-8185-z E-mail:ydlixiaoxia@yau.edu.cn
Supported by:
Supported by the Natural Science Foundation of Yan'an University, China(No.YDY2018-07).

摘要/Abstract

摘要： A highly sensitive and selective label-free electrochemical sensor was developed for the determination of kanamycin. To improve the sensitivity of the electrochemical sensor, graphene-gold nanoparticles were prepared by a one-step electrochemical coreduction process and were modified on the surface of a glassy carbon electrode. The double-stranded DNA(ds-DNA) duplex probe was immobilized onto the graphene-gold nanoparticle-modified electrode. The introduction of target kanamycin induced the displacement of aptamer from the ds-DNA duplex into the solution. Methylene blue(MB) as a redox indicator monitored the current change using differential pulse voltammetry. Under optimal conditions, the designed electrochemical aptasensor exhibited a wide linear range from 0.1 pmol/L to 10 pmol/L with a detection limit of 0.03 pmol/L for kanamycin. The experimental strategy enabled the direct analysis of milk samples, and the results showed high sensitivity and good selectivity.

关键词: Kanamycin, Electrochemical aptasensor, Graphene, Gold nanoparticle, Methylene blue

Abstract: A highly sensitive and selective label-free electrochemical sensor was developed for the determination of kanamycin. To improve the sensitivity of the electrochemical sensor, graphene-gold nanoparticles were prepared by a one-step electrochemical coreduction process and were modified on the surface of a glassy carbon electrode. The double-stranded DNA(ds-DNA) duplex probe was immobilized onto the graphene-gold nanoparticle-modified electrode. The introduction of target kanamycin induced the displacement of aptamer from the ds-DNA duplex into the solution. Methylene blue(MB) as a redox indicator monitored the current change using differential pulse voltammetry. Under optimal conditions, the designed electrochemical aptasensor exhibited a wide linear range from 0.1 pmol/L to 10 pmol/L with a detection limit of 0.03 pmol/L for kanamycin. The experimental strategy enabled the direct analysis of milk samples, and the results showed high sensitivity and good selectivity.

Key words: Kanamycin, Electrochemical aptasensor, Graphene, Gold nanoparticle, Methylene blue

ZHENG Jingyi, FENG Rongrong, HE Caimei and LI Xiaoxia. Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement[J]. 高等学校化学研究, 2018, 34(6): 952-958.

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Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement

Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement

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