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高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (6): 947-952.

• Articles • 上一篇    下一篇

Detection of Lectins by Saccharide-gold Nanoparticle Conjugates

HOU Qiong1,2, WANG Jin-e2, LIU Xia2, LI Xiao-kun2, MA Li-na2, DUAN Wu-biao1,3, WANG Zhen-xin2   

  1. 1. School of Chemistry, Heilongjiang University, Harbin 150080, P. R. China;
    2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
    3. School of Sciences, Beijing Jiaotong University, Beijing 100044, P. R. China
  • 收稿日期:2012-03-26 修回日期:2012-05-07 出版日期:2012-11-25 发布日期:2012-11-09
  • 通讯作者: DUAN Wu-biao, WANG Zhen-xin E-mail:duanwubiao2003@yahoo.com.cn;wangzx@ciac.jl.cn
  • 基金资助:

    Supported by the National Basic Research Program of China(No.2011CB935800) and the Project of the Jilin Provincial Science and Technology Department, China(No.20100701).

Detection of Lectins by Saccharide-gold Nanoparticle Conjugates

HOU Qiong1,2, WANG Jin-e2, LIU Xia2, LI Xiao-kun2, MA Li-na2, DUAN Wu-biao1,3, WANG Zhen-xin2   

  1. 1. School of Chemistry, Heilongjiang University, Harbin 150080, P. R. China;
    2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
    3. School of Sciences, Beijing Jiaotong University, Beijing 100044, P. R. China
  • Received:2012-03-26 Revised:2012-05-07 Online:2012-11-25 Published:2012-11-09
  • Contact: DUAN Wu-biao, WANG Zhen-xin E-mail:duanwubiao2003@yahoo.com.cn;wangzx@ciac.jl.cn
  • Supported by:

    Supported by the National Basic Research Program of China(No.2011CB935800) and the Project of the Jilin Provincial Science and Technology Department, China(No.20100701).

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摘要：

A general functionalization strategy was reported, which enables one to conjugate saccharide(SA) on gold nanoparticle(GNP) surface without affecting SA properties. First, disulfide phenylboronic acid(Bor) functionalized GNPs(Bor@GNPs) were synthesized by the reaction of citrate stabilized GNPs of 13 nm in diameter with the mixture of Bor and pentapeptide(Cys-Ala-Leu-Asn-Asn, CALNN). Subsequently, the SA-GNP conjugates(SA@GNPs) were prepared by coupling SA to the GNP surface via the reaction of phenylboronic acid(PBA) with the cis-diol configuration in SA. The interactions of three SA@GNPs with three lectins have been analyzed by UV-visible spectroscopic and transmission electronic microscopic(TEM) techniques, respectively. The experimental results demonstrate that SA@GNPs can efficiently bind to lectins and show a great promise as optical probes for monitoring specific affinities of lectins for SA, and detecting lectins with high sensitivity.

关键词: Saccharide, Gold nanoparticle, Lectin, Phenylboronic acid

Abstract:

A general functionalization strategy was reported, which enables one to conjugate saccharide(SA) on gold nanoparticle(GNP) surface without affecting SA properties. First, disulfide phenylboronic acid(Bor) functionalized GNPs(Bor@GNPs) were synthesized by the reaction of citrate stabilized GNPs of 13 nm in diameter with the mixture of Bor and pentapeptide(Cys-Ala-Leu-Asn-Asn, CALNN). Subsequently, the SA-GNP conjugates(SA@GNPs) were prepared by coupling SA to the GNP surface via the reaction of phenylboronic acid(PBA) with the cis-diol configuration in SA. The interactions of three SA@GNPs with three lectins have been analyzed by UV-visible spectroscopic and transmission electronic microscopic(TEM) techniques, respectively. The experimental results demonstrate that SA@GNPs can efficiently bind to lectins and show a great promise as optical probes for monitoring specific affinities of lectins for SA, and detecting lectins with high sensitivity.

Key words: Saccharide, Gold nanoparticle, Lectin, Phenylboronic acid