Preparation of β-Cyclodextrin Modified Fused Silica Capillary and Its Application in Capillary Electrophoresis Enantiomeric Separation

高等学校化学研究 ›› 1999, Vol. 15 ›› Issue (3): 232-237.

Preparation of β-Cyclodextrin Modified Fused Silica Capillary and Its Application in Capillary Electrophoresis Enantiomeric Separation

ZHANG Xin-xiang, HONG Feng, BAI Jing, CHANG Wen-bao, CI Yun-xiang

College of Chemistry, Peking University, Beijing 100871, P. R. China

收稿日期:1998-12-04 出版日期:1999-08-24 发布日期:2011-08-17
通讯作者: ZHANG Xin-xiang
基金资助:
Supported by the National Natural Science Foundation of China(No.29305035)and SRFROCS from State Education Ministry.

Preparation of β-Cyclodextrin Modified Fused Silica Capillary and Its Application in Capillary Electrophoresis Enantiomeric Separation

ZHANG Xin-xiang, HONG Feng, BAI Jing, CHANG Wen-bao, CI Yun-xiang

College of Chemistry, Peking University, Beijing 100871, P. R. China

Received:1998-12-04 Online:1999-08-24 Published:2011-08-17
Supported by:
Supported by the National Natural Science Foundation of China(No.29305035)and SRFROCS from State Education Ministry.

摘要/Abstract

摘要： A simple type of chiral selector immobilized capillaries was prepared and applied to the separation of some enantiomers by capillary electrophoresis(CE) without chiral selective reagents in a buffer solution. β-Cyclodextrin (CD) was bonded to the inner wall of fused silica capillary with the aid of 3-(2-cyclooxypropoxyl)-propyl-trimethoxy silane or 3-aminopropyltriethoxyl silane as bridge. The synthesis conditions such as reaction temperature, reaction ratios, solvents were optimized. The stability and reproducibility of the CD-modified capillaries were studied under different operation conditions. It is satisfactory to apply the capillary to the chiral separation of d,l-adrenaline and the atracurium besilate diastereoisomers under the optimized conditions.

关键词: &beta, -cyclodextrin-immobilized capillary, Capillary electrophoresis, Enantiomeric separation

Abstract: A simple type of chiral selector immobilized capillaries was prepared and applied to the separation of some enantiomers by capillary electrophoresis(CE) without chiral selective reagents in a buffer solution. β-Cyclodextrin (CD) was bonded to the inner wall of fused silica capillary with the aid of 3-(2-cyclooxypropoxyl)-propyl-trimethoxy silane or 3-aminopropyltriethoxyl silane as bridge. The synthesis conditions such as reaction temperature, reaction ratios, solvents were optimized. The stability and reproducibility of the CD-modified capillaries were studied under different operation conditions. It is satisfactory to apply the capillary to the chiral separation of d,l-adrenaline and the atracurium besilate diastereoisomers under the optimized conditions.

Key words: β-cyclodextrin-immobilized capillary, Capillary electrophoresis, Enantiomeric separation

ZHANG Xin-xiang, HONG Feng, BAI Jing, CHANG Wen-bao, CI Yun-xiang . Preparation of β-Cyclodextrin Modified Fused Silica Capillary and Its Application in Capillary Electrophoresis Enantiomeric Separation[J]. 高等学校化学研究, 1999, 15(3): 232-237.

[1]	XU Jia, YU Xiaoxuan, ZHANG Yue, LIU Naizhang, GUAN Shuwen, WANG Liping. Extending Lifespan of Alzheimer's Mode Nematode CL4176 Using a Novel Bifunctional Peptide with Inhibition of β-Amyloid Aggregation and Anti-oxidation[J]. 高等学校化学研究, 2019, 35(2): 245-250.
[2]	WEI Wei, ZHAO Chaoyue, LIU Sitong, JIAO Xiaofei, LIU Xintong, LAN Chuanjian, LI Jiang. Expression of Phytoestrogens in pGL2/AQP1 Promoter Reporter System[J]. 高等学校化学研究, 2018, 34(6): 929-933.
[3]	LI Guohua, LI Huipeng, ZHAO Hua, CAI Tianfeng, LI Yange and GUAN Shengnan. Acylation of Anisole Catalyzed by Hierarchical Porous Hβ Zeolite Modified with Cr[J]. 高等学校化学研究, 2018, 34(6): 877-881.
[4]	KANG Nan, CHEN Zhanguo. Base-catalyzed Formation of Enamines from β,β-Dicyanostyrene Derivatives with N-Bromosaccharin[J]. 高等学校化学研究, 2018, 34(5): 751-757.
[5]	DONG Xu, ZHANG Chunxia, XU Jian, ZHANG Yue, ZHAO Ying, CHEN Li. Formation of β-Lactones by[2+2] Cycloaddition of Dichloroketene with Unreactive Carbonyl Compounds[J]. 高等学校化学研究, 2018, 34(5): 736-743.
[6]	ZHANG Di, TAN Liming, DONG Jia, YI Jiaqiang, WANG Peng, ZHANG Jianping. Structure and Excitation Dynamics of β-Carotene Aggregates in Cetyltrimethylammonium Bromide Micelle[J]. 高等学校化学研究, 2018, 34(4): 643-648.
[7]	SONG Shengmei, LI Minglu, GONG Xiaojuan, HAN Hui, ZHOU Yehong, WANG Li, SHUANG Shaomin, DONG Chuan. Controlled Release of Curcumin via Folic Acid Conjugated Magnetic Drug Delivery System[J]. 高等学校化学研究, 2018, 34(2): 203-211.
[8]	LI Yuwei, BU Mingwei, CHEN Peng, LI Xiaohong, CHEN Changwu, GAO Gui, FENG Yan, HAN Weiwei, ZHANG Zuoming. Characterization of a Thermophilic Monosaccharide Stimulated β-Glucosidase from Acidothermus cellulolyticus[J]. 高等学校化学研究, 2018, 34(2): 212-220.
[9]	SI Qingbo, WEN Qian, YANG Qingbiao, SONG Yan, LI Yaoxian. Preparation of β-Cyclodextrin/Fe₃O₄/Polyvinylpyrrolidone Composite Magnetic Microspheres for the Adsorption of Methyl Orange[J]. 高等学校化学研究, 2017, 33(6): 1012-1016.
[10]	CHENG Dandan, WEI Wei, LI Yuxin, HUA Xuewen, ZHOU Yunyun, LIU Jingbo, LI Yonghong, YU Shujing, XIONG Lixia, LI Zhengming. Design, Synthesis and Biological Activities of Novel 4,5-Disubstituted-3-S-(β-D-acetylglycosyl)-1,2,4-triazole Derivatives[J]. 高等学校化学研究, 2017, 33(5): 758-764.
[11]	ZHANG Lei, LI Hongfeng, CHEN Peng, SUN Wenbin, YAN Pengfei. Quadruple-stranded Eu-helicate Assembled from Bis-β-diketonate: Its Stability Towards Metal Ions[J]. 高等学校化学研究, 2016, 32(4): 534-538.
[12]	CHEN Bin, CHEN Jie, YANG Liming, ZHAO Guochen, DING Guowei. Functional β-Cyclodextrin Coating by Electrodeposition on Stainless Steel for Drug Loading and Release[J]. 高等学校化学研究, 2016, 32(2): 278-283.
[13]	SONG Shengmei, MA Xuewen, ZHOU Yehong, XU Maotian, SHUANG Shaomin, DONG Chuan. Studies on the Interaction Between Vanillin and β-Amyloid Protein via Fluorescence Spectroscopy and Atomic Force Microscopy[J]. 高等学校化学研究, 2016, 32(2): 172-177.
[14]	CHEN Zhanguo, DU Manfei, XIA Wei, HU Junli. One-pot Synthesis of α,β-Dehydroamino Derivatives from β,β-Dicyanostyrene with 1,3-Dibromo-5,5-dimethylhydantoin Promoted by Mild Base[J]. 高等学校化学研究, 2016, 32(1): 68-75.
[15]	ZHENG Chunying, QIU Qiming, HAO Liang, LI Hui. Studies on the Halogen Substituted β-Amino Acids and Their Cu(Ⅱ) Coordination Complexes in Crystallography[J]. 高等学校化学研究, 2016, 32(1): 1-7.

Preparation of β-Cyclodextrin Modified Fused Silica Capillary and Its Application in Capillary Electrophoresis Enantiomeric Separation

Preparation of β-Cyclodextrin Modified Fused Silica Capillary and Its Application in Capillary Electrophoresis Enantiomeric Separation

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价