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高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (6): 986-990.

• Articles • 上一篇    下一篇

Preliminary Research on a Mesenchymal Stem Cell-model for Screening Traditional Chinese Medicines

FAN Xue-mei1, LI Xue2, WANG Yi-ming3, LIANG Qiong-lin3 and LUO Guo-an1,3*   

  1. 1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, P. R. China;
    2. School of Medicine,
    3. Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China
  • 收稿日期:2009-10-28 修回日期:2010-04-23 出版日期:2010-11-25 发布日期:2010-12-01
  • 通讯作者: LUO Guo-an E-mail:luoga@tsinghua.edu.cn
  • 作者简介:LUO Guo-an. E-mail: luoga@tsinghua.edu.cn
  • 基金资助:

    Supported by the National Eleventh Five-Year Plan of China(No.2006BA108B04-01) and the National Basic Research Program of China(No.2005CB523503).

Preliminary Research on a Mesenchymal Stem Cell-model for Screening Traditional Chinese Medicines

FAN Xue-mei1, LI Xue2, WANG Yi-ming3, LIANG Qiong-lin3 and LUO Guo-an1,3*   

  1. 1. School of Pharmacy, East China University of Science and Technology, Shanghai 200237, P. R. China;
    2. School of Medicine,
    3. Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China
  • Received:2009-10-28 Revised:2010-04-23 Online:2010-11-25 Published:2010-12-01
  • Contact: LUO Guo-an E-mail:luoga@tsinghua.edu.cn
  • About author:LUO Guo-an. E-mail: luoga@tsinghua.edu.cn
  • Supported by:

    Supported by the National Eleventh Five-Year Plan of China(No.2006BA108B04-01) and the National Basic Research Program of China(No.2005CB523503).

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摘要： The authors focused their attention on the establishment of a mesenchymal stem cell(MSC) model for screening traditional Chinese medicines(TCMs) so as to investigate the effects of Shuanglong Formula(SLF) components(Ginsenosides and salvianolic acids) and ingredients(ginsenoside Rb1 and salvianolic acid B) on cardiomyocyte differentiation from MSCs. The SLF components were analyzed and quantified by HPLC-TOF-MS. Cardiomyocyte differentiation was induced by culturing MSCs in the induction medium supplemented with SLF ingredients，SLF components, 5-azacytidine(5-aza), 5-aza+SLF ingredients and 5-aza+SLF components, respectively, for up to 30 d, and evulated by the expression of Cardiac-specific myosin heavy chain(MHC) and troponin I(TnI) via immunofluoresent staining. Slow growth rate and changed morphology were observed during cardiomyocyte differentiation. After 20 d of induction, differentiating MSCs were positive for MHC and TnI staining. The effects of SLF components were better than those of SLF ingredients. Taken together, SLF can induce the differentiation of MSCs into cardiomyogenic cells in vitro, and MSCs can be used as a powerful tool for screening TCMs.

关键词: Bone marrow mesenchymal stem cell, Screening model, Shuanglong Formula, Immunofluoresent staining

Abstract: The authors focused their attention on the establishment of a mesenchymal stem cell(MSC) model for screening traditional Chinese medicines(TCMs) so as to investigate the effects of Shuanglong Formula(SLF) components(Ginsenosides and salvianolic acids) and ingredients(ginsenoside Rb1 and salvianolic acid B) on cardiomyocyte differentiation from MSCs. The SLF components were analyzed and quantified by HPLC-TOF-MS. Cardiomyocyte differentiation was induced by culturing MSCs in the induction medium supplemented with SLF ingredients，SLF components, 5-azacytidine(5-aza), 5-aza+SLF ingredients and 5-aza+SLF components, respectively, for up to 30 d, and evulated by the expression of Cardiac-specific myosin heavy chain(MHC) and troponin I(TnI) via immunofluoresent staining. Slow growth rate and changed morphology were observed during cardiomyocyte differentiation. After 20 d of induction, differentiating MSCs were positive for MHC and TnI staining. The effects of SLF components were better than those of SLF ingredients. Taken together, SLF can induce the differentiation of MSCs into cardiomyogenic cells in vitro, and MSCs can be used as a powerful tool for screening TCMs.

Key words: Bone marrow mesenchymal stem cell, Screening model, Shuanglong Formula, Immunofluoresent staining