An Improved Method of Simultaneous Determination of Four Bioactive Compounds in Evodiae Fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography

doi:10.1007/s40242-017-6434-1

高等学校化学研究 ›› 2017, Vol. 33 ›› Issue (4): 552-558.doi: 10.1007/s40242-017-6434-1

An Improved Method of Simultaneous Determination of Four Bioactive Compounds in Evodiae Fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography

BIAN Min, TIAN Ling, YAO Cheng

College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211800, P. R. China

收稿日期:2016-10-31 修回日期:2017-03-11 出版日期:2017-08-01 发布日期:2017-07-19
通讯作者: BIAN Min E-mail:bianmin8024@163.com
基金资助:
Supported by the National Natural Science Foundation of China（No.21405082）.

An Improved Method of Simultaneous Determination of Four Bioactive Compounds in Evodiae Fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography

BIAN Min, TIAN Ling, YAO Cheng

College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing 211800, P. R. China

Received:2016-10-31 Revised:2017-03-11 Online:2017-08-01 Published:2017-07-19
Contact: BIAN Min E-mail:bianmin8024@163.com
Supported by:
Supported by the National Natural Science Foundation of China（No.21405082）.

摘要/Abstract

摘要：

There are many compounds with different structures and chemical properties in Evodiae Fructus. It is hard to simultaneously determine the bioactive compounds by high performance liquid chromatography(HPLC). A new method was proposed for four bioactive compounds(synephrine, limonoids, evodiamine and rutecarpine) to be separated completely and determined accurately using ionic liquids(ILs) as mobile phase additives. The mechanism and the effect of the ILs for changing the chromatographic behaviors of the four compounds were studied by systematically changing the pH value of mobile phase, the types and concentrations of ILs as well as the concentrations of phosphate buffer. The chromatographic behaviors of the analytes with a mobile phase containing ILs complied with the stoichiometric displacement model for retention(SDM-R). All results demonstrate the dual nature of ionic liquids, which are competitive adsorption and ion-pair agent. Meanwhile, excellent linearity was observed for all the compounds with correlation coefficients between 0.9992 and 0.9998. The limit of detection and the limit of quantification of the four compounds varied from 0.47 μg/mL to 0.87 μg/mL and from 1.79 μg/mL to 2.44 μg/mL, respectively. Three kinds of Evodiae Fructus processed through different methods were analyzed via the method. The result shows that the contents of evodiamine and rutecarpine as the two main active compounds by processing with vinegar and salt are obviously higher than those of the raw products.

关键词: Evodiae Fructus, Ionic liquid, Mobile phase additive, High performance liquid chromatography(HPLC), Processed Evodiae Fructus

Abstract:

Key words: Evodiae Fructus, Ionic liquid, Mobile phase additive, High performance liquid chromatography(HPLC), Processed Evodiae Fructus

BIAN Min, TIAN Ling, YAO Cheng. An Improved Method of Simultaneous Determination of Four Bioactive Compounds in Evodiae Fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography[J]. 高等学校化学研究, 2017, 33(4): 552-558.

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An Improved Method of Simultaneous Determination of Four Bioactive Compounds in Evodiae Fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography

An Improved Method of Simultaneous Determination of Four Bioactive Compounds in Evodiae Fructus Using Ionic Liquids as Mobile Phase Additives in High Performance Liquid Chromatography

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