Au Nanoparticle-DNAzyme Dual Catalyst System for Sensitively Colorimetric Detection of Thrombin

doi:10.1007/s40242-013-3039-1

高等学校化学研究 ›› 2013, Vol. 29 ›› Issue (5): 868-873.doi: 10.1007/s40242-013-3039-1

Au Nanoparticle-DNAzyme Dual Catalyst System for Sensitively Colorimetric Detection of Thrombin

RAO Xin-yi¹, ZHANG Jia-jia¹, CUI Jing¹, HU Ying¹, LIU Ting¹, CHAI Jing-feng², CHENG Gui-fang¹, HE Pin-gang¹, FANG Yu-zhi¹

1. Department of Chemistry, East China Normal University, Shanghai 200241, P. R. China;
2. Shanghai International Airport Co., Ltd., Shanghai 201202, P. R. China

收稿日期:2013-01-21 修回日期:2013-05-09 出版日期:2013-10-01 发布日期:2013-09-17
通讯作者: CHENG Gui-fang E-mail:gfcheng@chem.ecnu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(No.21175045) and the Project of Shanghai Science and Technology Committee, China(No.10ZR1409400).

Au Nanoparticle-DNAzyme Dual Catalyst System for Sensitively Colorimetric Detection of Thrombin

RAO Xin-yi¹, ZHANG Jia-jia¹, CUI Jing¹, HU Ying¹, LIU Ting¹, CHAI Jing-feng², CHENG Gui-fang¹, HE Pin-gang¹, FANG Yu-zhi¹

1. Department of Chemistry, East China Normal University, Shanghai 200241, P. R. China;
2. Shanghai International Airport Co., Ltd., Shanghai 201202, P. R. China

Received:2013-01-21 Revised:2013-05-09 Online:2013-10-01 Published:2013-09-17
Contact: CHENG Gui-fang E-mail:gfcheng@chem.ecnu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21175045) and the Project of Shanghai Science and Technology Committee, China(No.10ZR1409400).

摘要/Abstract

摘要：

A novel colorimetric aptasensor was developed for thrombin detection with high sensitivity and specificity. The assay takes the advantage of Au nanoparticles-DNAzyme as a dual catalytic system for signal amplification. Au nanparticles were modified with peroxidase mimicking DNAzyme sequence as well as thrombin binding aptamer. And then the thrombin binding aptamer hybridized with its complementary sequence which was immobilized on the surface of the magnetic nanoparticles to construct the colorimetric aptasensor. In the presence of thrombin, the target-induced displacement takes place, resulting in the dissociation of the aptasensor. The DNAzyme functioned Au NPs are released due to the combine ability of thrombin binding aptamer with thrombin. The released Au NPs are capable of catalyzing the colorless 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonic acid(ABTS) conversion into a blue-green product by H₂O₂-mediated oxidation, thus can amplify the colorimetric readout signals of thrombin detection. Such a device can serve as a novel selectivity sensor for thrombin with a detection limit of 0.6 nmol/L.

关键词: Au nanoparticle, Magnetic nanoparticle, DNAzyme, Aptamer, Thrombin

Abstract:

Key words: Au nanoparticle, Magnetic nanoparticle, DNAzyme, Aptamer, Thrombin

RAO Xin-yi, ZHANG Jia-jia, CUI Jing, HU Ying, LIU Ting, CHAI Jing-feng, CHENG Gui-fang, HE Pin-gang, FANG Yu-zhi. Au Nanoparticle-DNAzyme Dual Catalyst System for Sensitively Colorimetric Detection of Thrombin[J]. 高等学校化学研究, 2013, 29(5): 868-873.

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Au Nanoparticle-DNAzyme Dual Catalyst System for Sensitively Colorimetric Detection of Thrombin

Au Nanoparticle-DNAzyme Dual Catalyst System for Sensitively Colorimetric Detection of Thrombin

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