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高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (2): 195-199 .

• Articles • 上一篇    下一篇

Investigation of Breaking Efficiency of N-Glycosidic Bond Between Sulfonamide and Honey

WANG Yan-hua1, LI Li1, CHEN Chia-hung2, CHENG Shuenn-ren3, LEE Mu-sheng2,5, YU Pu-ping4, CHANG-CHIEN Guo-ping2   

  1. 1. College of Biology and Enviornmental Engineering, Zhejiang Shuren University, Hangzhou 310015, P. R. China;
    2. Super Micro Mass Research & Technology Center, Cheng Shiu University, Kaohsiung 833, Taiwan, P. R. China;
    3. Department of Business Administration, Cheng Shiu University, Kaohsiung 833, Taiwan, P. R. China;
    4. Department of Mechanical Engineering, Cheng Shiu University, Kaohsiung 833, Taiwan, P. R. China;
    5. Environmental Protection Bureau Kaohsiung City Government, Keelung 20143, Taiwan, P. R. China
  • 收稿日期:2011-05-26 修回日期:2011-11-08 出版日期:2012-03-25 发布日期:2012-03-08
  • 通讯作者: CHANG-CHIEN Guo-ping E-mail:guoping@csu.edu.tw
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.20907046) and the Natural Science Foundation of Zhejiang Province of China(No.Y4100623).

Investigation of Breaking Efficiency of N-Glycosidic Bond Between Sulfonamide and Honey

WANG Yan-hua1, LI Li1, CHEN Chia-hung2, CHENG Shuenn-ren3, LEE Mu-sheng2,5, YU Pu-ping4, CHANG-CHIEN Guo-ping2   

  1. 1. College of Biology and Enviornmental Engineering, Zhejiang Shuren University, Hangzhou 310015, P. R. China;
    2. Super Micro Mass Research & Technology Center, Cheng Shiu University, Kaohsiung 833, Taiwan, P. R. China;
    3. Department of Business Administration, Cheng Shiu University, Kaohsiung 833, Taiwan, P. R. China;
    4. Department of Mechanical Engineering, Cheng Shiu University, Kaohsiung 833, Taiwan, P. R. China;
    5. Environmental Protection Bureau Kaohsiung City Government, Keelung 20143, Taiwan, P. R. China
  • Received:2011-05-26 Revised:2011-11-08 Online:2012-03-25 Published:2012-03-08
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20907046) and the Natural Science Foundation of Zhejiang Province of China(No.Y4100623).

PDF (PC)

摘要： Sulfonamide residue in honey existed in a form bonded to sugar via the N-glycosidic bond. It would result in the possible underestimation of concentration of sulfonamide if it is not decomposed by chemical methods. However, in Mainland China and Taiwan(P. R. China), the regulation for sulfonamide residue analysis does not include hydrolysis and has been applied to a very broad range of samples, for example, egg, milk, meat, seafood as well as honey. This paper demonstrates the necessity of hydrolysis of it prior to extracting honey. The breaking efficiencies of N-glycosidic bond were investigated by 2 mol/L hydrochloric acid, pure methanol and 0.5 mol/L hydrochloric acid in methanol, respectively. It was found that acid plays the key role in breaking the N-glycosidic bond, and it was also noticed that the dissolution of liberated sulfonamide in methanol could carry the reaction forward in favor of breaking the N-glycosidic bond.

关键词: Sulfonamide, Honey, Liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI- MS/MS), N-Glycosidic bond

Abstract: Sulfonamide residue in honey existed in a form bonded to sugar via the N-glycosidic bond. It would result in the possible underestimation of concentration of sulfonamide if it is not decomposed by chemical methods. However, in Mainland China and Taiwan(P. R. China), the regulation for sulfonamide residue analysis does not include hydrolysis and has been applied to a very broad range of samples, for example, egg, milk, meat, seafood as well as honey. This paper demonstrates the necessity of hydrolysis of it prior to extracting honey. The breaking efficiencies of N-glycosidic bond were investigated by 2 mol/L hydrochloric acid, pure methanol and 0.5 mol/L hydrochloric acid in methanol, respectively. It was found that acid plays the key role in breaking the N-glycosidic bond, and it was also noticed that the dissolution of liberated sulfonamide in methanol could carry the reaction forward in favor of breaking the N-glycosidic bond.

Key words: Sulfonamide, Honey, Liquid chromatography-electrospray ionization tandem mass spectrometry(LC-ESI- MS/MS), N-Glycosidic bond