A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP

高等学校化学研究 ›› 1993, Vol. 9 ›› Issue (4): 314-316.

A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP

ZHANG Zhu-jun, ZHANG Su-sen, ZHANG Xin-rong

Department of Chemistry, Shaanxi Normal University, Xian, 710062

收稿日期:1993-03-03 出版日期:1993-12-24 发布日期:2011-08-17
通讯作者: ZHANG Zhu-jun
基金资助:
Supported by the National Natural Science Foundation of China

A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP

ZHANG Zhu-jun, ZHANG Su-sen, ZHANG Xin-rong

Department of Chemistry, Shaanxi Normal University, Xian, 710062

Received:1993-03-03 Online:1993-12-24 Published:2011-08-17
Supported by:
Supported by the National Natural Science Foundation of China

摘要/Abstract

摘要： A new chemiluminescence amplification reaction has been developed, which is on the coupling of the oxidation of Eosin by hydrogen peroxide under the catalysis of HRP at pH 3. 5 to the chemiluminescent reaction of luminol and hydrogen peroxide catalyzed by HRP at pH 10 to measure the activity of HRP and labelled-HRP. The detection limit of HRP is 10^-10 g/mL. The lowest dilution ratio of HBsAb-HRP is 1 : 1. 8×10² and 1 : 10⁶ for the direct method and coupled method respectively.

关键词: Chemiluminescence, HRP, Labelled HRP, Determination

Abstract: A new chemiluminescence amplification reaction has been developed, which is on the coupling of the oxidation of Eosin by hydrogen peroxide under the catalysis of HRP at pH 3. 5 to the chemiluminescent reaction of luminol and hydrogen peroxide catalyzed by HRP at pH 10 to measure the activity of HRP and labelled-HRP. The detection limit of HRP is 10^-10 g/mL. The lowest dilution ratio of HBsAb-HRP is 1 : 1. 8×10² and 1 : 10⁶ for the direct method and coupled method respectively.

Key words: Chemiluminescence, HRP, Labelled HRP, Determination

ZHANG Zhu-jun, ZHANG Su-sen, ZHANG Xin-rong . A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP[J]. 高等学校化学研究, 1993, 9(4): 314-316.

ZHANG Zhu-jun, ZHANG Su-sen, ZHANG Xin-rong . A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP[J]. Chemical Research in Chinese Universities, 1993, 9(4): 314-316.

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A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP

A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP

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