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高等学校化学研究 ›› 2006, Vol. 22 ›› Issue (4): 474-478.

• Articles • 上一篇    下一篇

Biosensor Based on TTF@SiO2 Nanoparticles as Electron Transfer Mediator for the Determination of Glucose

LI Xiao-hua, XIAN Yue-zhong, MIN Hong, LI Chen-xin, XIE Zong-hong, ZHANG Fen-fen, JIN Li-tong   

  1. Department of Chemistry, East China Normal University, Shanghai 200062, P. R. China
  • 收稿日期:2005-10-08 出版日期:2006-08-24 发布日期:2011-08-06
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No. 20305007) and Doctoral Foundation of China Ministry of Education(No. 20030269014).

Biosensor Based on TTF@SiO2 Nanoparticles as Electron Transfer Mediator for the Determination of Glucose

LI Xiao-hua, XIAN Yue-zhong, MIN Hong, LI Chen-xin, XIE Zong-hong, ZHANG Fen-fen, JIN Li-tong   

  1. Department of Chemistry, East China Normal University, Shanghai 200062, P. R. China
  • Received:2005-10-08 Online:2006-08-24 Published:2011-08-06
  • Supported by:

    Supported by the National Natural Science Foundation of China(No. 20305007) and Doctoral Foundation of China Ministry of Education(No. 20030269014).

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摘要： Tetrathiafulvalene(TTF) was doped in an SiO2 network and the resulting nanocomposite was used as a mediator for the selective detection of glucose. The uniform TTF-doped silica(TTF@SiO2) nanoparticles were prepared by the water-in-oil(W/O) microemulsion method, and were characterized by transmission electron microscopy(TEM). The core-shell structure TTF@SiO2 could prevent TTF from leaching out into an aqueous solution. Combined with chitosan(CHIT), which serves as a scaffold for glucose oxidase and nanocomposite immobilization, the GCE/TTF@SiO2-CHIT-GOx biosensor was developed. Under optimal conditions, the biosensors exhibit a linear range of 1.0×10-5-5×10-3 mol/L with a detection limit down to 5.0 μmol/L(S/N=3). The excellent selectivity, sensitivity, and stability of the glucose biosensor show its potential for practical applications.

关键词: TTF@SiO2 nanoparticle, Chitosan, Glucose, Biosensor

Abstract: Tetrathiafulvalene(TTF) was doped in an SiO2 network and the resulting nanocomposite was used as a mediator for the selective detection of glucose. The uniform TTF-doped silica(TTF@SiO2) nanoparticles were prepared by the water-in-oil(W/O) microemulsion method, and were characterized by transmission electron microscopy(TEM). The core-shell structure TTF@SiO2 could prevent TTF from leaching out into an aqueous solution. Combined with chitosan(CHIT), which serves as a scaffold for glucose oxidase and nanocomposite immobilization, the GCE/TTF@SiO2-CHIT-GOx biosensor was developed. Under optimal conditions, the biosensors exhibit a linear range of 1.0×10-5-5×10-3 mol/L with a detection limit down to 5.0 μmol/L(S/N=3). The excellent selectivity, sensitivity, and stability of the glucose biosensor show its potential for practical applications.

Key words: TTF@SiO2 nanoparticle, Chitosan, Glucose, Biosensor