Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

doi:10.1007/s40242-023-3026-0

高等学校化学研究 ›› 2023, Vol. 39 ›› Issue (6): 992-997.doi: 10.1007/s40242-023-3026-0

Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

QIAO Juan^1,2, ZHANG Xinya^1,3, CHENG Cheng^1,4, and QI Li^1,2

1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China;
2. School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, P.R. China;
3. School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, P.R. China;
4. College of Chemistry & Environmental Science, Hebei University, Baoding 071002, P.R. China

收稿日期:2023-02-07 出版日期:2023-12-01 发布日期:2023-11-18
通讯作者: QI Li E-mail:qili@iccas.ac.cn
基金资助:
This work was supported by the National Natural Science Foundation of China (No.21727809).

Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

QIAO Juan^1,2, ZHANG Xinya^1,3, CHENG Cheng^1,4, and QI Li^1,2

1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China;
2. School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, P.R. China;
3. School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, P.R. China;
4. College of Chemistry & Environmental Science, Hebei University, Baoding 071002, P.R. China

Received:2023-02-07 Online:2023-12-01 Published:2023-11-18
Contact: QI Li E-mail:qili@iccas.ac.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (No.21727809).

摘要/Abstract

摘要： Immobilization of enzymes onto porous membranes has attracted considerable attention in recent years. However, enhancing the enzymolysis efficiency of the resulting enzyme reactors by varying the environmental conditions poses a great challenge. In this work, poly(styrene-maleic anhydride-N,N-dimethylacrylamide) was prepared and utilized to construct a thermo-sensitive porous polymer membrane-based enzyme reactor (TS-PPMER) after cellulase was immobilized onto the support by covalent bonding. The catalytic activity of the nano-reactor was evaluated by measuring the yield of the product, glucose, at different temperatures with carboxymethylcellulose as the substrate. Interestingly, the polymer chains coiled and formed numerous nano-pores at a high temperature, which induced the confine effect and greatly boosted the enzymolysis efficiency of TS-PPMER. Furthermore, the proposed TS-PPMER was applied in the hydrolysis of green plant leaves in Epipremnum aureum. This work shows great potential in obtaining biological resources by an environmentally friendly approach using smart polymer-based nano-reactors.

关键词: Thermo-sensitive porous polymer membrane, Cellulase reactor, Catalytic efficiency, Glucose, Plant leaf

Abstract: Immobilization of enzymes onto porous membranes has attracted considerable attention in recent years. However, enhancing the enzymolysis efficiency of the resulting enzyme reactors by varying the environmental conditions poses a great challenge. In this work, poly(styrene-maleic anhydride-N,N-dimethylacrylamide) was prepared and utilized to construct a thermo-sensitive porous polymer membrane-based enzyme reactor (TS-PPMER) after cellulase was immobilized onto the support by covalent bonding. The catalytic activity of the nano-reactor was evaluated by measuring the yield of the product, glucose, at different temperatures with carboxymethylcellulose as the substrate. Interestingly, the polymer chains coiled and formed numerous nano-pores at a high temperature, which induced the confine effect and greatly boosted the enzymolysis efficiency of TS-PPMER. Furthermore, the proposed TS-PPMER was applied in the hydrolysis of green plant leaves in Epipremnum aureum. This work shows great potential in obtaining biological resources by an environmentally friendly approach using smart polymer-based nano-reactors.

Key words: Thermo-sensitive porous polymer membrane, Cellulase reactor, Catalytic efficiency, Glucose, Plant leaf

QIAO Juan, ZHANG Xinya, CHENG Cheng, and QI Li. Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature[J]. 高等学校化学研究, 2023, 39(6): 992-997.

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Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

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