Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection

doi:10.1007/s40242-021-1038-1

高等学校化学研究 ›› 2022, Vol. 38 ›› Issue (2): 529-534.doi: 10.1007/s40242-021-1038-1

Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection

LAN Weifei¹, HU Ruifeng², HUANG Danrong¹, DONG Xu¹, SHEN Gangyi¹, CHANG Shan³, DAI Dongsheng³

1. School of Pharmacy, Minzu University of China, Beijing 100081, P. R. China;
2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
3. Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, P. R. China

收稿日期:2021-01-29 修回日期:2021-04-19 出版日期:2022-04-01 发布日期:2021-04-19
通讯作者: SHEN Gangyi, DAI Dongsheng E-mail:shengy@muc.edu.cn;dongshengdai@126.com
基金资助:
This work was supported by the National Natural Science Foundation of China(No.81573834).

Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection

LAN Weifei¹, HU Ruifeng², HUANG Danrong¹, DONG Xu¹, SHEN Gangyi¹, CHANG Shan³, DAI Dongsheng³

1. School of Pharmacy, Minzu University of China, Beijing 100081, P. R. China;
2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
3. Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213001, P. R. China

Received:2021-01-29 Revised:2021-04-19 Online:2022-04-01 Published:2021-04-19
Contact: SHEN Gangyi, DAI Dongsheng E-mail:shengy@muc.edu.cn;dongshengdai@126.com
Supported by:
This work was supported by the National Natural Science Foundation of China(No.81573834).

摘要/Abstract

摘要： In this study, palladium nanoparticles loaded graphdiyne oxide (Pd/GDYO) nanocomposite were fabricated by in-situ reduction of palladium chloride in the dispersion of GDYO, and characte-rized by Raman spectra, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The synthesized Pd/GDYO was first found to have catalytic activities similar to those of the peroxidase enzyme, which can catalyze the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine(TMB) in the presence of hydrogen peroxide(H₂O₂). Steady-state kinetic studies showed that the catalytic reaction of Pd/GDYO follows a ping-pong mechanism, and Pd/GDYO has a stronger activity to TMB with a Michaelis constant(K_m) value of 5.32×10^-4 mmol/L. Based on the shielding effect of glutathione(GSH) on the Pd/GDYO-H₂O₂-TMB reaction system, a colorimetric detection method for GSH was deve-loped with a wide linear range from 0.1 μmol/L to 40 μmol/L and a limit of detection of 0.1 μmol/L. The method was successfully applied for fast and accurate detection of GSH in injection powder drugs. It was expected that this peroxidase-like Pd/GDYO nano- composite would have wide applications in the fields of biomedicine and environmental chemistry.

关键词: Palladium nanoparticle/graphdiyne oxide nanocomposite, Peroxidase-like activity, Colorimetric detection, Glutathione

Abstract: In this study, palladium nanoparticles loaded graphdiyne oxide (Pd/GDYO) nanocomposite were fabricated by in-situ reduction of palladium chloride in the dispersion of GDYO, and characte-rized by Raman spectra, transmission electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy. The synthesized Pd/GDYO was first found to have catalytic activities similar to those of the peroxidase enzyme, which can catalyze the oxidation of peroxidase substrate 3,3',5,5'-tetramethylbenzidine(TMB) in the presence of hydrogen peroxide(H₂O₂). Steady-state kinetic studies showed that the catalytic reaction of Pd/GDYO follows a ping-pong mechanism, and Pd/GDYO has a stronger activity to TMB with a Michaelis constant(K_m) value of 5.32×10^-4 mmol/L. Based on the shielding effect of glutathione(GSH) on the Pd/GDYO-H₂O₂-TMB reaction system, a colorimetric detection method for GSH was deve-loped with a wide linear range from 0.1 μmol/L to 40 μmol/L and a limit of detection of 0.1 μmol/L. The method was successfully applied for fast and accurate detection of GSH in injection powder drugs. It was expected that this peroxidase-like Pd/GDYO nano- composite would have wide applications in the fields of biomedicine and environmental chemistry.

Key words: Palladium nanoparticle/graphdiyne oxide nanocomposite, Peroxidase-like activity, Colorimetric detection, Glutathione

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LAN Weifei, HU Ruifeng, HUANG Danrong, DONG Xu, SHEN Gangyi, CHANG Shan, DAI Dongsheng. Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection[J]. 高等学校化学研究, 2022, 38(2): 529-534.

LAN Weifei, HU Ruifeng, HUANG Danrong, DONG Xu, SHEN Gangyi, CHANG Shan, DAI Dongsheng. Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection[J]. Chemical Research in Chinese Universities, 2022, 38(2): 529-534.

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链接本文: https://crcu.jlu.edu.cn/CN/10.1007/s40242-021-1038-1

https://crcu.jlu.edu.cn/CN/Y2022/V38/I2/529

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[10] WANG Cheng, WAN Pei, GONG Ping-sheng, Lü Li-min, XU Ya-wei, ZHAO Yang, HE Bo, ZH. Novel Selenium-containing Human Single-chain Variable Fragment with Glutathione Peroxidase Activity from Computer-aided Molecular Design[J]. 高等学校化学研究, 2011, 27(5): 813-819.

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[1]	SHI Rui, WEI Shuxian, CHENG Shiqi, ZENG Jinmin, WANG Yilin, SHU Xin. Colorimetric Detection of Glucose Using WO₃ Nanosheets as Peroxidase-mimetic Enzyme[J]. 高等学校化学研究, 2022, 38(4): 985-990.
[2]	SU Ce, BAI Lingling, ZHANG Hongbo, CHANG Kaishan, LI Guanbin, LI Siliang. Novel Approach for the Decoration of Magnetic Carbon Nanospheres with Platinum Nanoparticles and Their Enhanced Peroxidase Activity for the Colorimetric Detection of H₂O₂[J]. 高等学校化学研究, 2019, 35(1): 163-170.
[3]	XU Gaoping, TANG Yonghe, LIN Weiying. Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells[J]. 高等学校化学研究, 2018, 34(4): 523-527.
[4]	SU Jing, YU Mingyue, LIN Zhen, ZHAO Hang, LIU Haiyan. Effects of ZnO Nanoparticles on Rat Glioma C6 Cells[J]. 高等学校化学研究, 2017, 33(6): 908-911.
[5]	ZHANG Ya, QIAN Linlin, YIN Wei, HE Bin, LIU Fangmei, HOU Changjun, HUO Danqun, FA Huanbao. A Dual Read-out Molecularly Imprinted Composite Membrane Sensor Based on Zinc Porphyrin for the Detection of Dimethyl Methylphosphonate[J]. 高等学校化学研究, 2016, 32(5): 725-730.
[6]	SHEN Na, YAN Fei, LÜ Shaowu, YANG Wenkui, LUO Guimin, YAN Ganglin, MU Ying. Gene Manipulation Based Selenium-containing Peptide Exhibiting Synergism of SOD and GPx[J]. 高等学校化学研究, 2014, 30(6): 947-952.
[7]	YIN Li, HAN Xiao, YU Yang, GUO Xiao, REN Li-qun, FANG Jing-qi, LIU Zhi-yi, YAN Gang-lin, WEI Jing-yan. Antioxidant Effect of Selenium-containing Glutathione S-Transferase in Rat Cardiomyocytes[J]. 高等学校化学研究, 2012, 28(3): 454-458.
[8]	LI Xue, WU Yun-jian, LI Zhuo, CHU Wen-ting, ZHANG Hong-xing, ZHENG Qing-chuan. Theoretical Study on GSH Activation Mechanism of a New Type of Glutathione Transferase Gtt2[J]. 高等学校化学研究, 2012, 28(3): 500-502.
[9]	HOU Ju-ying and AI Shi-yun*. Electrochemical Determination of Reduced Glutathione at Multiwalled Carbon Nanotubes/Poly(bromocresol green) Modified Glassy Carbon Electrode[J]. 高等学校化学研究, 2011, 27(6): 934-938.
[10]	WANG Cheng, WAN Pei, GONG Ping-sheng, Lü Li-min, XU Ya-wei, ZHAO Yang, HE Bo, ZH. Novel Selenium-containing Human Single-chain Variable Fragment with Glutathione Peroxidase Activity from Computer-aided Molecular Design[J]. 高等学校化学研究, 2011, 27(5): 813-819.
[11]	LU Guang-hua, CHEN Wei*, LI Ying and ZHU Zhi. Effects of PAHs on Biotransformation Enzymatic Activities in Fish[J]. 高等学校化学研究, 2011, 27(3): 413-416.
[12]	SHEN Na, YAN Fei, GUO Yi, Lü Shao-wu, GONG Ping-sheng, XU Ya-wei, .... Imprinted Human Serum Albumin with Antioxidant Activity[J]. 高等学校化学研究, 2011, 27(2): 258-263.
[13]	SHAO Peng, YUAN Xing, LIU Rui and CAO Jian-ping. Effects of Nitrobenzene on Liver Antioxidant Defense System of Carassius auratus[J]. 高等学校化学研究, 2010, 26(2): 204-209.
[14]	HUO Rui, SHI Yi, XU Jun-jie, YAN Fei, LU Shao-wu, SU Jia-ming, DUAN Yu-jing, FAN Jia, NING Bo, CONG Deng-li, YAN Gang-lin, LUO Gui-min , WEI Jing-yan. Antioxidant Effect of Human Selenium-containing Single-chain Fv in Rat Cardiac Myocytes[J]. 高等学校化学研究, 2009, 25(2): 216-219.
[15]	SUN Ye, LI Wei-jia, MA Ji-sheng, MU Ying, DU Xiu-bo, YAN Gang-lin, LUO Gui-min. Soluble Expression, Purification and Characterization of Single-chain Fv Catalytic Antibody(sFv-2F3)[J]. 高等学校化学研究, 2004, 20(3): 317-322.

Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection

Palladium Nanoparticles/Graphdiyne Oxide Nanocomposite with Excellent Peroxidase-like Activity and Its Application for Glutathione Detection

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