Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells

doi:10.1007/s40242-018-8089-y

高等学校化学研究 ›› 2018, Vol. 34 ›› Issue (4): 523-527.doi: 10.1007/s40242-018-8089-y

Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells

XU Gaoping, TANG Yonghe, LIN Weiying

Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China

收稿日期:2018-03-20 出版日期:2018-08-01 发布日期:2018-05-21
通讯作者: LIN Weiying E-mail:weiyinglin2013@163.com
基金资助:
Supported by the National Natural Science Foundation of China(Nos.21472067, 21672083), the Taishan Scholar Foundation, China(No.TS20151104 1) and the Startup Fund of University of Jinan, China(No.309-10004).

Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells

XU Gaoping, TANG Yonghe, LIN Weiying

Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, P. R. China

Received:2018-03-20 Online:2018-08-01 Published:2018-05-21
Contact: LIN Weiying E-mail:weiyinglin2013@163.com
Supported by:
Supported by the National Natural Science Foundation of China(Nos.21472067, 21672083), the Taishan Scholar Foundation, China(No.TS20151104 1) and the Startup Fund of University of Jinan, China(No.309-10004).

摘要/Abstract

摘要： Glutathione(GSH), as the most abundant intracellular biothiol, plays an important role in the redox homeostasis of the organism. Abnormal concentrations of GSH in cells may lead to many malignant diseases, such as cancer, liver damage and neurodegenerative diseases. It is urgent to develop effective methods to detect GSH in living organisms. In this work, a new two-photon ratiometric fluorescent probe Co-GSH based on the coumarin-chalcone dye platform was judiciously developed. Based on the Michael-addition reaction, Co-GSH was able to identify GSH with high selectivity and sensitivity. Furthermore, assisted by laser-scanning confocal microscopy, Co-GSH could specifically response GSH over the other biothiols, including Cys and Hcy, in living HeLa cells by using one-and two-photon modes.

关键词: Fluorescence probe, Glutathione, Michael-addition reaction, Two-photon, Ratiometric

Abstract: Glutathione(GSH), as the most abundant intracellular biothiol, plays an important role in the redox homeostasis of the organism. Abnormal concentrations of GSH in cells may lead to many malignant diseases, such as cancer, liver damage and neurodegenerative diseases. It is urgent to develop effective methods to detect GSH in living organisms. In this work, a new two-photon ratiometric fluorescent probe Co-GSH based on the coumarin-chalcone dye platform was judiciously developed. Based on the Michael-addition reaction, Co-GSH was able to identify GSH with high selectivity and sensitivity. Furthermore, assisted by laser-scanning confocal microscopy, Co-GSH could specifically response GSH over the other biothiols, including Cys and Hcy, in living HeLa cells by using one-and two-photon modes.

Key words: Fluorescence probe, Glutathione, Michael-addition reaction, Two-photon, Ratiometric

XU Gaoping, TANG Yonghe, LIN Weiying. Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells[J]. 高等学校化学研究, 2018, 34(4): 523-527.

XU Gaoping, TANG Yonghe, LIN Weiying. Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells[J]. Chemical Research in Chinese Universities, 2018, 34(4): 523-527.

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Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells

Development of a Two-photon Ratiometric Fluorescent Probe for Glutathione and Its Applications in Living Cells

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