Effect of a Short Peptide with Alternating L- and D-Amino Acid on the Aggregation and Membrane Damage of hIAPP

doi:10.1007/s40242-021-0386-1

高等学校化学研究 ›› 2021, Vol. 37 ›› Issue (3): 787-794.doi: 10.1007/s40242-021-0386-1

Effect of a Short Peptide with Alternating L- and D-Amino Acid on the Aggregation and Membrane Damage of hIAPP

MENG Feihong, WANG Yajie, LU Tong, WANG Chunyu, LI Fei

State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, P. R. China

收稿日期:2020-11-22 修回日期:2020-12-14 出版日期:2021-06-01 发布日期:2020-12-18
通讯作者: LI Fei E-mail:feili@jlu.edu.cn
基金资助:
This work was supported by the National Natural Science Foundation of China(No.21673099).

Effect of a Short Peptide with Alternating L- and D-Amino Acid on the Aggregation and Membrane Damage of hIAPP

MENG Feihong, WANG Yajie, LU Tong, WANG Chunyu, LI Fei

State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012, P. R. China

Received:2020-11-22 Revised:2020-12-14 Online:2021-06-01 Published:2020-12-18
Contact: LI Fei E-mail:feili@jlu.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China(No.21673099).

摘要/Abstract

摘要： Alpha-sheet is believed to be a significant structural component, formed in the fibrillation process of the amyloid peptide. However, the knowledge about the role of α-sheet played in the amyloidosis and toxicity is lack. In this work, we modified a short peptide derived from the core region of human islet amyloid polypetide(hIAPP, hIAPP_18-27) with an alternating D-amino acid replacement and investigated the effects of the L/D alternating peptide on the fibrillar aggregation and the membrane damage of hIAPP using NMR, ThT fluorescence assay, circular dichroism(CD), transmission electron microscopy(TEM) and leakage assay, and compared the results with those of hIAPP_18-27without D-amino acid replacement. We show that the short peptide with alternating L- and D-amino acids forms an α-sheet structure and is more potent in promoting the fibrillation of hIAPP and reducing the ability of hIAPP to disrupt the membrane composed of POPG and POPC[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine] 1:4 lipids than the short peptide with all L-amino acids in a random coil structure. The higher potency of the D/L alternating peptide in these activities is attributed to its ability to induce the α-sheet-like structure in the core region of hIAPP and block the interaction of hIAPP with the membrane more effectively.

关键词: Alpha-sheet, Amyloid peptide, Toxicity

Abstract: Alpha-sheet is believed to be a significant structural component, formed in the fibrillation process of the amyloid peptide. However, the knowledge about the role of α-sheet played in the amyloidosis and toxicity is lack. In this work, we modified a short peptide derived from the core region of human islet amyloid polypetide(hIAPP, hIAPP_18-27) with an alternating D-amino acid replacement and investigated the effects of the L/D alternating peptide on the fibrillar aggregation and the membrane damage of hIAPP using NMR, ThT fluorescence assay, circular dichroism(CD), transmission electron microscopy(TEM) and leakage assay, and compared the results with those of hIAPP_18-27without D-amino acid replacement. We show that the short peptide with alternating L- and D-amino acids forms an α-sheet structure and is more potent in promoting the fibrillation of hIAPP and reducing the ability of hIAPP to disrupt the membrane composed of POPG and POPC[1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine] 1:4 lipids than the short peptide with all L-amino acids in a random coil structure. The higher potency of the D/L alternating peptide in these activities is attributed to its ability to induce the α-sheet-like structure in the core region of hIAPP and block the interaction of hIAPP with the membrane more effectively.

Key words: Alpha-sheet, Amyloid peptide, Toxicity

MENG Feihong, WANG Yajie, LU Tong, WANG Chunyu, LI Fei. Effect of a Short Peptide with Alternating L- and D-Amino Acid on the Aggregation and Membrane Damage of hIAPP[J]. 高等学校化学研究, 2021, 37(3): 787-794.

MENG Feihong, WANG Yajie, LU Tong, WANG Chunyu, LI Fei. Effect of a Short Peptide with Alternating L- and D-Amino Acid on the Aggregation and Membrane Damage of hIAPP[J]. Chemical Research in Chinese Universities, 2021, 37(3): 787-794.

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Effect of a Short Peptide with Alternating L- and D-Amino Acid on the Aggregation and Membrane Damage of hIAPP

Effect of a Short Peptide with Alternating L- and D-Amino Acid on the Aggregation and Membrane Damage of hIAPP

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