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高等学校化学研究 ›› 2016, Vol. 32 ›› Issue (5): 792-796.doi: 10.1007/s40242-016-6122-6

• Articles • 上一篇    下一篇

Isolation and Stucture Elucidation of Diterpenes from Wedelia prostrata and Their Cytotoxicity Activities

CHEN Qing, LIN Jianhong, ZHAO Dan, ZHU Xuan   

  1. Department of Pharmacy, School of Pharmaceutical Science, Xiamen University, Xiamen 361102, P. R. China
  • 收稿日期:2016-03-30 修回日期:2016-05-30 出版日期:2016-10-01 发布日期:2016-09-24
  • 通讯作者: ZHU Xuan E-mail:zhuxuan@xmu.edu.cn
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.31300285), the Natural Science Foundation of Fujian Province, China(No.2015J01348), the Specialized Research Fund for the Doctoral Program of Higher Education of China (No.20110073120072) and the Fujian Province Development Guidance(Key) Project, China(No.2014Y0074).

Isolation and Stucture Elucidation of Diterpenes from Wedelia prostrata and Their Cytotoxicity Activities

CHEN Qing, LIN Jianhong, ZHAO Dan, ZHU Xuan   

  1. Department of Pharmacy, School of Pharmaceutical Science, Xiamen University, Xiamen 361102, P. R. China
  • Received:2016-03-30 Revised:2016-05-30 Online:2016-10-01 Published:2016-09-24
  • Contact: ZHU Xuan E-mail:zhuxuan@xmu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.31300285), the Natural Science Foundation of Fujian Province, China(No.2015J01348), the Specialized Research Fund for the Doctoral Program of Higher Education of China (No.20110073120072) and the Fujian Province Development Guidance(Key) Project, China(No.2014Y0074).

摘要:

In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance(NMR) techniques and mass spectrometry and identified to be 3α-phenylpropionoyloxy-ent-kaur-16-en-oic acid(1). The isolated diterpenes were tested for their cytotoxicity activities via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines(IC50=6.14 and 8.76 μmol/L).

关键词: Wedelia prostrata, 3α-Phenylpropionoyloxy-ent-kaur-16-en-oic acid, Chemical constituent, Cytotoxicity activity

Abstract:

In this paper, a new diterpene together with seven known diterpenes was isolated from Wedelia prostrata. The chemical structure of the new compound was elucidated via 1D and 2D nuclear magnetic resonance(NMR) techniques and mass spectrometry and identified to be 3α-phenylpropionoyloxy-ent-kaur-16-en-oic acid(1). The isolated diterpenes were tested for their cytotoxicity activities via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. The results show that compounds 1, 2, 3, 4 and 6 exhibit different levels of cytotoxic activities. Especially, compound 2 shows significant cytotoxicity toward HeLa and A549 cell lines(IC50=6.14 and 8.76 μmol/L).

Key words: Wedelia prostrata, 3α-Phenylpropionoyloxy-ent-kaur-16-en-oic acid, Chemical constituent, Cytotoxicity activity