Chemical Synthesis of Proteins Containing 300 Amino Acids

doi:10.1007/s40242-020-0150-y

高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (5): 733-747.doi: 10.1007/s40242-020-0150-y

Chemical Synthesis of Proteins Containing 300 Amino Acids

ZHANG Baochang, LI Yulei, SHI Weiwei, WANG Tongyue, ZHANG Feng, LIU Lei

Tsinghua-Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology(Ministry of Education), Center for Synthetic and Systems Biology, State Key Laboratory of Chemical Oncogenomics(Shenzhen), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China

收稿日期:2020-05-12 修回日期:2020-06-02 出版日期:2020-10-01 发布日期:2020-10-01
通讯作者: LIU Lei E-mail:lliu@mail.tsinghua.edu.cn
基金资助:
Supported by the National Key R&D Program of China(No.2017YFA0505200) and the National Natural Science Foundation of China(Nos.21532004, 91753205, 81621002).

Chemical Synthesis of Proteins Containing 300 Amino Acids

ZHANG Baochang, LI Yulei, SHI Weiwei, WANG Tongyue, ZHANG Feng, LIU Lei

Tsinghua-Peking Center for Life Sciences, Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology(Ministry of Education), Center for Synthetic and Systems Biology, State Key Laboratory of Chemical Oncogenomics(Shenzhen), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China

Received:2020-05-12 Revised:2020-06-02 Online:2020-10-01 Published:2020-10-01
Contact: LIU Lei E-mail:lliu@mail.tsinghua.edu.cn
Supported by:
Supported by the National Key R&D Program of China(No.2017YFA0505200) and the National Natural Science Foundation of China(Nos.21532004, 91753205, 81621002).

摘要/Abstract

摘要： Chemical synthesis of proteins containing up to 300 amino acids may cover 30%-50% of all the proteins encountered in biomedical studies and may provide an alternate approach to the usually used recombinant expression technology, vastly expanding the chemical space of the latter. In the present review article, we tried to survey the recent progresses made for more rapid synthesis of increasingly long peptides and more efficient ligation of multiple peptide segments. The developments of seminal methods by many research groups have greatly contributed to the recent breakthroughs in the successful total synthesis of a number of functionally important proteins, such as oligoubiquitins, bacterial GroEL/ES chaperones, and mirror-image DNA polymerases. Through these studies, a potential bottleneck has also been recognized for the chemical synthesis of large proteins, namely, how to ensure that each peptide segment from a large protein avoids unfavorable aggregation when dissolved in aqueous solution. Many new methods, such as removable backbone modification(RBM) strategy have been developed to overcome this bottleneck, while more studies need to be carried out to develop more effective and less costly methods that ultimately, may lead to fully automatable chemical synthesis of customized proteins of 300 amino acids bearing any artificial designs.

关键词: Chemical protein synthesis, Solid-phase synthesis, Peptide, Ligation, 300 amino acid

Abstract: Chemical synthesis of proteins containing up to 300 amino acids may cover 30%-50% of all the proteins encountered in biomedical studies and may provide an alternate approach to the usually used recombinant expression technology, vastly expanding the chemical space of the latter. In the present review article, we tried to survey the recent progresses made for more rapid synthesis of increasingly long peptides and more efficient ligation of multiple peptide segments. The developments of seminal methods by many research groups have greatly contributed to the recent breakthroughs in the successful total synthesis of a number of functionally important proteins, such as oligoubiquitins, bacterial GroEL/ES chaperones, and mirror-image DNA polymerases. Through these studies, a potential bottleneck has also been recognized for the chemical synthesis of large proteins, namely, how to ensure that each peptide segment from a large protein avoids unfavorable aggregation when dissolved in aqueous solution. Many new methods, such as removable backbone modification(RBM) strategy have been developed to overcome this bottleneck, while more studies need to be carried out to develop more effective and less costly methods that ultimately, may lead to fully automatable chemical synthesis of customized proteins of 300 amino acids bearing any artificial designs.

Key words: Chemical protein synthesis, Solid-phase synthesis, Peptide, Ligation, 300 amino acid

ZHANG Baochang, LI Yulei, SHI Weiwei, WANG Tongyue, ZHANG Feng, LIU Lei. Chemical Synthesis of Proteins Containing 300 Amino Acids[J]. 高等学校化学研究, 2020, 36(5): 733-747.

ZHANG Baochang, LI Yulei, SHI Weiwei, WANG Tongyue, ZHANG Feng, LIU Lei. Chemical Synthesis of Proteins Containing 300 Amino Acids[J]. Chemical Research in Chinese Universities, 2020, 36(5): 733-747.

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Chemical Synthesis of Proteins Containing 300 Amino Acids

Chemical Synthesis of Proteins Containing 300 Amino Acids

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