DNA Nanostructures as Drug Carriers for Cellular Delivery

doi:10.1007/s40242-020-9070-0

高等学校化学研究 ›› 2020, Vol. 36 ›› Issue (2): 177-184.doi: 10.1007/s40242-020-9070-0

DNA Nanostructures as Drug Carriers for Cellular Delivery

WU Na, ZHAO Yongxi

Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China

收稿日期:2019-11-28 修回日期:2019-12-30 出版日期:2020-04-01 发布日期:2020-01-13
通讯作者: ZHAO Yongxi E-mail:yxzhao@mail.xjtu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(Nos.31700861, 21804105, 31671013), the Natural Science Foundation of Shannxi Province, China(No.S2018-JC-JQ-0037), the Fundamental Research Funds for the Central Universities of China(Nos.xjj2018083, xjj2018257) and the "Young Talent Support Plan" of Xi'an Jiaotong University, China.

DNA Nanostructures as Drug Carriers for Cellular Delivery

WU Na, ZHAO Yongxi

Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P. R. China

Received:2019-11-28 Revised:2019-12-30 Online:2020-04-01 Published:2020-01-13
Contact: ZHAO Yongxi E-mail:yxzhao@mail.xjtu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.31700861, 21804105, 31671013), the Natural Science Foundation of Shannxi Province, China(No.S2018-JC-JQ-0037), the Fundamental Research Funds for the Central Universities of China(Nos.xjj2018083, xjj2018257) and the "Young Talent Support Plan" of Xi'an Jiaotong University, China.

摘要/Abstract

摘要： Drug delivery systems have been widely developed for enhancing target activity and improving drug functions. Liposomes, high-molecular polymer, gold nanoparticles and carbon nanomaterials, etc., are all the candidates of drug carriers. However, immunotoxicity, heterogeneity and low solubility generally exist and hamper their applications. As a kind of biological materials, DNA has its unique advantages in biomedical applications, including excellent biological compatibility and programmability. DNA nanostructures have been proved to possess high cellular uptake efficiency, which sheds new light on DNA-based drug delivery system. In this review, we summarize the influence factors of DNA nanostructure internalization efficiency, including cell lines, and the size and the shape of DNA nanoparticles. Uniformity of DNA nanostructures in appearance and properties ensures the stability in research, which makes DNA carriers stand out from other nanomaterials. Next, we focus on the functionalization of DNA carriers, which endows DNA nanostructures with the potential to construct integrated drug delivery platforms. We also discuss the internalization pathways of DNA nanostructures and their fate in cells. The deeply understanding about the endocytic pathways provides new sight for the further design strategy on changing the transportation routes of DNA carriers in cells. Finally, the challenges in further applications are discussed, and suggestions are proposed.

关键词: DNA nanostructure, Drug delivery, Cellular uptake, Internalization pathway, DNA-based drug carrier

Abstract: Drug delivery systems have been widely developed for enhancing target activity and improving drug functions. Liposomes, high-molecular polymer, gold nanoparticles and carbon nanomaterials, etc., are all the candidates of drug carriers. However, immunotoxicity, heterogeneity and low solubility generally exist and hamper their applications. As a kind of biological materials, DNA has its unique advantages in biomedical applications, including excellent biological compatibility and programmability. DNA nanostructures have been proved to possess high cellular uptake efficiency, which sheds new light on DNA-based drug delivery system. In this review, we summarize the influence factors of DNA nanostructure internalization efficiency, including cell lines, and the size and the shape of DNA nanoparticles. Uniformity of DNA nanostructures in appearance and properties ensures the stability in research, which makes DNA carriers stand out from other nanomaterials. Next, we focus on the functionalization of DNA carriers, which endows DNA nanostructures with the potential to construct integrated drug delivery platforms. We also discuss the internalization pathways of DNA nanostructures and their fate in cells. The deeply understanding about the endocytic pathways provides new sight for the further design strategy on changing the transportation routes of DNA carriers in cells. Finally, the challenges in further applications are discussed, and suggestions are proposed.

Key words: DNA nanostructure, Drug delivery, Cellular uptake, Internalization pathway, DNA-based drug carrier

WU Na, ZHAO Yongxi. DNA Nanostructures as Drug Carriers for Cellular Delivery[J]. 高等学校化学研究, 2020, 36(2): 177-184.

WU Na, ZHAO Yongxi. DNA Nanostructures as Drug Carriers for Cellular Delivery[J]. Chemical Research in Chinese Universities, 2020, 36(2): 177-184.

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DNA Nanostructures as Drug Carriers for Cellular Delivery

DNA Nanostructures as Drug Carriers for Cellular Delivery

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