Preparation of Surface-enhanced Raman Scattering(SERS)-active Optical Fiber Sensor by Laser-induced Ag Deposition and Its Application in Bioidentification of Biotin/Avidin

doi:10.1007/s40242-015-4294-0

高等学校化学研究 ›› 2015, Vol. 31 ›› Issue (1): 25-30.doi: 10.1007/s40242-015-4294-0

Preparation of Surface-enhanced Raman Scattering(SERS)-active Optical Fiber Sensor by Laser-induced Ag Deposition and Its Application in Bioidentification of Biotin/Avidin

LIU Chunyu^1,2, WANG Shaoyan¹, FU Cuicui¹, LI Haibo¹, XU Shuping¹, XU Weiqing¹

1. State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, P. R. China;
2. College of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China

收稿日期:2014-08-01 修回日期:2014-09-03 出版日期:2015-02-01 发布日期:2014-09-22
通讯作者: XU Weiqing, E-mail:xuwq@jlu.edu.cn E-mail:xuwq@jlu.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(Nos.91027010, 21073073, 20903043, 20973075), the Research Fund for the Doctoral Program of Higher Education of China(No.20090061120089) and the National Instrumentation Program(NIP) of the Ministry of Science and Technology of China(No.2011YQ03012408).

Preparation of Surface-enhanced Raman Scattering(SERS)-active Optical Fiber Sensor by Laser-induced Ag Deposition and Its Application in Bioidentification of Biotin/Avidin

LIU Chunyu^1,2, WANG Shaoyan¹, FU Cuicui¹, LI Haibo¹, XU Shuping¹, XU Weiqing¹

1. State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, P. R. China;
2. College of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China

Received:2014-08-01 Revised:2014-09-03 Online:2015-02-01 Published:2014-09-22
Contact: XU Weiqing, E-mail:xuwq@jlu.edu.cn E-mail:xuwq@jlu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.91027010, 21073073, 20903043, 20973075), the Research Fund for the Doctoral Program of Higher Education of China(No.20090061120089) and the National Instrumentation Program(NIP) of the Ministry of Science and Technology of China(No.2011YQ03012408).

摘要/Abstract

摘要：

A surface-enhanced Raman scattering(SERS) optical fiber sensor was prepared by the laser-induced deposition of Ag nanoparticle membrane on a silica optical fiber tip, which was applied to the real time SERS spectral monitoring on the biorecognition of biotin/avidin. The bioidentification of biotin/avidin was carried out through a indirect method, in which the bioidentification is based on the SERS response signal of a labeled dye(Atto610) after its fluorescence has been quenched totally by the deposited Ag nanoparticle membrane. By SERS monitoring the bioidentification process of biotin/avidin, it has been found that this recognition process is finished in 40 min. The lowest detection concentration of biotin is 1.0×10^-7 mg/mL. This research is promising in the application of immunoassays on line and in vivo.

关键词: Process monitoring, Photochemical method, Laser-inducement, SERS-active optical fiber, Biotin/avidin, Specific identification

Abstract:

Key words: Process monitoring, Photochemical method, Laser-inducement, SERS-active optical fiber, Biotin/avidin, Specific identification

LIU Chunyu, WANG Shaoyan, FU Cuicui, LI Haibo, XU Shuping, XU Weiqing. Preparation of Surface-enhanced Raman Scattering(SERS)-active Optical Fiber Sensor by Laser-induced Ag Deposition and Its Application in Bioidentification of Biotin/Avidin[J]. 高等学校化学研究, 2015, 31(1): 25-30.

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Preparation of Surface-enhanced Raman Scattering(SERS)-active Optical Fiber Sensor by Laser-induced Ag Deposition and Its Application in Bioidentification of Biotin/Avidin

Preparation of Surface-enhanced Raman Scattering(SERS)-active Optical Fiber Sensor by Laser-induced Ag Deposition and Its Application in Bioidentification of Biotin/Avidin

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