Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

doi:10.1007/s40242-013-2300-y

高等学校化学研究 ›› 2013, Vol. 29 ›› Issue (2): 290-293.doi: 10.1007/s40242-013-2300-y

Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

LI Wen-gui¹, DAI Fei-yan¹, CHENG Yong-xian², YIN Ge-fen¹, BI Jun-long¹, LI Dian-peng³

1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, P. R. China;
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, P. R. China;
3. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin 541006, P. R. China

收稿日期:2012-08-13 修回日期:2012-10-15 出版日期:2013-04-01 发布日期:2013-03-20
通讯作者: CHENG Yong-xian E-mail:yxcheng@mail.kib.ac.cn
基金资助:
Supported by the Project of Talent Scholarship for the Youth of Yunnan Province of China(No.2007PY01-48), the Sino- German International Collaboration Project from Yunnan Province of China(No.2009AC011), the Project of Natural Compound Library Construction from Chinese Academy of Sciences(No.KSCX2-EW-R-15) and the Yunnan Provincial Program for Excellent Scientists, China(No.2009CI125).

Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

LI Wen-gui¹, DAI Fei-yan¹, CHENG Yong-xian², YIN Ge-fen¹, BI Jun-long¹, LI Dian-peng³

1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, P. R. China;
2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, P. R. China;
3. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin 541006, P. R. China

Received:2012-08-13 Revised:2012-10-15 Online:2013-04-01 Published:2013-03-20
Supported by:
Supported by the Project of Talent Scholarship for the Youth of Yunnan Province of China(No.2007PY01-48), the Sino- German International Collaboration Project from Yunnan Province of China(No.2009AC011), the Project of Natural Compound Library Construction from Chinese Academy of Sciences(No.KSCX2-EW-R-15) and the Yunnan Provincial Program for Excellent Scientists, China(No.2009CI125).

摘要/Abstract

摘要：

Porcine reproductive and respiratory syndrome(PRRS) caused by porcine reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious diseases in the swine industry worldwide, causing big economic losses. Vaccines are major weapons against PRRSV, however, current available vaccines have several limitations. Developing chemical drugs as alternatives is required. On the basis of traditional medical knowledge, we purposely selected 15 natural products originated from Chinese herbs with anti-infectious effects. Their antiviral activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 cells and reverse transcription polymerase chain reaction(RT-PCR) assay. Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits which could significantly reduce PRRSV-associated CPE with 50% inhibited concentration(IC₅₀) values of 7.9 and 39.4 mmol/L, respectively. Meanwhile, compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA expression of ORF7 gene in a dose-dependent manner. Study results suggest that compounds ethoxy- sanguinarine and atractylodinol may be useful anti-PRRSV drugs for swine industry or the hits for further lead optimization.

关键词: Porcine reproductive and respiratory syndrome virus(PRRSV), Natural product, Cytopathic effect, Antiviral activity

Abstract:

Key words: Porcine reproductive and respiratory syndrome virus(PRRSV), Natural product, Cytopathic effect, Antiviral activity

LI Wen-gui, DAI Fei-yan, CHENG Yong-xian, YIN Ge-fen, BI Jun-long, LI Dian-peng. Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products[J]. 高等学校化学研究, 2013, 29(2): 290-293.

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Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

Identification of Porcine Reproductive and Respiratory Syndrome Virus Inhibitors Through an Oriented Screening on Natural Products

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