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高等学校化学研究 ›› 2012, Vol. 28 ›› Issue (5): 856-861 .

• Articles • 上一篇    下一篇

Knockdown of STAT3 by iRNA Inhibiting Migration and Invasion of Epithelial Ovarian Cancer Cells

LI Qin-hua1, ZHU Ji-hong1, LIU Lei2, YUE Ying1   

  1. 1. Department of Gynecology, the First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. Department of Gastroenterology,the First Hospital of Jilin University, Changchun 130021, P. R. China
  • 收稿日期:2011-12-02 修回日期:2012-03-07 出版日期:2012-09-25 发布日期:2012-09-07
  • 通讯作者: YUE Ying E-mail:yying119@126.com
  • 基金资助:
    Supported by the Basis of Bethune Medical Research Fund of Jilin Province of China(No.200705114).

Knockdown of STAT3 by iRNA Inhibiting Migration and Invasion of Epithelial Ovarian Cancer Cells

LI Qin-hua1, ZHU Ji-hong1, LIU Lei2, YUE Ying1   

  1. 1. Department of Gynecology, the First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. Department of Gastroenterology,the First Hospital of Jilin University, Changchun 130021, P. R. China
  • Received:2011-12-02 Revised:2012-03-07 Online:2012-09-25 Published:2012-09-07
  • Supported by:
    Supported by the Basis of Bethune Medical Research Fund of Jilin Province of China(No.200705114).

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摘要： Signal transducer and activator of transcription 3(STAT3) is a dual functional transcription factor with the functions of signal transduction and transcription regulation. It is reported that the expression of STAT3 in ovarian cancer is significantly higher and STAT3 can facilitate ovarian cancer growth and metastasis. To clarify the definite effect and molecular mechanism of STAT3 involved in ovarian cancer growth and metastasis, STAT3 expression was significantly downregulated by transfecting ovarian cancer model SK-OV-3 cells with the plasmid vector which express specific RNAi that targets human STAT3. The downregulated STAT3 not only decreased the invasion and migration but also inhibited the proliferation of SK-OV-3 cells. Western blot assay shows that the expression of vascular endothelial growth factor(VEGF) and that of Survivin were reduced in the cells with the plasma vector expressing specific RNAi that targets human STAT3. These results demonstrate that STAT3 involved in the invasion and migration of SK-OV-3 regulates the expression of VEGF and Survivin. In addition, VEGF and Survivin could play an important role in ovarian cancer growth and metastasis.

关键词: Ovarian cancel model SK-OV-3 cell, Signal transducer and activator of transcription 3(STAT3), Migration, Vascular endothelial growth factor(VEGF), Survivin

Abstract: Signal transducer and activator of transcription 3(STAT3) is a dual functional transcription factor with the functions of signal transduction and transcription regulation. It is reported that the expression of STAT3 in ovarian cancer is significantly higher and STAT3 can facilitate ovarian cancer growth and metastasis. To clarify the definite effect and molecular mechanism of STAT3 involved in ovarian cancer growth and metastasis, STAT3 expression was significantly downregulated by transfecting ovarian cancer model SK-OV-3 cells with the plasmid vector which express specific RNAi that targets human STAT3. The downregulated STAT3 not only decreased the invasion and migration but also inhibited the proliferation of SK-OV-3 cells. Western blot assay shows that the expression of vascular endothelial growth factor(VEGF) and that of Survivin were reduced in the cells with the plasma vector expressing specific RNAi that targets human STAT3. These results demonstrate that STAT3 involved in the invasion and migration of SK-OV-3 regulates the expression of VEGF and Survivin. In addition, VEGF and Survivin could play an important role in ovarian cancer growth and metastasis.

Key words: Ovarian cancel model SK-OV-3 cell, Signal transducer and activator of transcription 3(STAT3), Migration, Vascular endothelial growth factor(VEGF), Survivin