Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS

高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (3): 371-374.

Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS

LI Guo-chen¹ , WANG Yan-hong^1*, WU Ren-an² and WANG Shi-cheng¹

1. Inspection and Testing Center of Agro-product Safety and Environment Quality, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China;
2. National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China

收稿日期:2010-09-16 修回日期:2010-12-28 出版日期:2011-05-25 发布日期:2011-04-29
通讯作者: WANG Yan-hong E-mail:wangyh@iae.ac.cn
基金资助:
Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(No.KSCX2-YW-N-035) and Projects of Shenyang City Science and Technology Burea, China(Nos.F10-224-4-00, 1091239-6-00).

Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS

LI Guo-chen¹ , WANG Yan-hong^1*, WU Ren-an² and WANG Shi-cheng¹

1. Inspection and Testing Center of Agro-product Safety and Environment Quality, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. China;
2. National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, P. R. China

Received:2010-09-16 Revised:2010-12-28 Online:2011-05-25 Published:2011-04-29
Contact: WANG Yan-hong E-mail:wangyh@iae.ac.cn
Supported by:
Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(No.KSCX2-YW-N-035) and Projects of Shenyang City Science and Technology Burea, China(Nos.F10-224-4-00, 1091239-6-00).

摘要/Abstract

摘要： The interest in the analysis of phospholipids(PLs), especially phosphatidylcholine(PC), has been increa- sing due to the importance of them in biochemistry as well as in industry. A method was reported based on the offline combination of MALDI-TOF MS and normal-phase HPLC for analyzing PLs extracted from crucian carp. Total PLs of crucian carp were extracted and then separated by HPLC before the collected subfractions were analyzed by MALDI-TOF MS. The mass spectra obtained show peaks of H+, Na+ and K+ adducts of PC molecules. It is shown that the prior separation of PLs by HPLC is highly necessary to remove the signal suppressing and to avoid the possible overlapping. With this method, 9 possible PC molecules in crucian carp and the corresponding fatty acid compositions were given from the well-resolved mass spectra.

关键词: Phospholipid, Phosphatidylcholine, Phosphatidylethanolamine, Sphingomyelin, HPLC, MALDI, Crucian Carp

Abstract: The interest in the analysis of phospholipids(PLs), especially phosphatidylcholine(PC), has been increa- sing due to the importance of them in biochemistry as well as in industry. A method was reported based on the offline combination of MALDI-TOF MS and normal-phase HPLC for analyzing PLs extracted from crucian carp. Total PLs of crucian carp were extracted and then separated by HPLC before the collected subfractions were analyzed by MALDI-TOF MS. The mass spectra obtained show peaks of H+, Na+ and K+ adducts of PC molecules. It is shown that the prior separation of PLs by HPLC is highly necessary to remove the signal suppressing and to avoid the possible overlapping. With this method, 9 possible PC molecules in crucian carp and the corresponding fatty acid compositions were given from the well-resolved mass spectra.

Key words: Phospholipid, Phosphatidylcholine, Phosphatidylethanolamine, Sphingomyelin, HPLC, MALDI, Crucian Carp

中图分类号:

O657.63

LI Guo-chen , WANG Yan-hong*, WU Ren-an and WANG Shi-cheng. Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS[J]. 高等学校化学研究, 2011, 27(3): 371-374.

LI Guo-chen , WANG Yan-hong*, WU Ren-an and WANG Shi-cheng. Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS[J]. Chemical Research in Chinese Universities, 2011, 27(3): 371-374.

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Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS

Analysis of Phospholipids in Crucian Carp(Carassius auratus) Muscle by Offline HPLC-MALDI-TOF MS

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