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高等学校化学研究 ›› 2011, Vol. 27 ›› Issue (1): 38-44.

• Articles • 上一篇    下一篇

Qualitative and Quantitative Analysis of Alkaloids in Cortex Phellodendri by HPLC-ESI-MS/MS and HPLC-DAD

ZHU Shuang-lai1,2, DOU Sheng-shan1, LIU Xin-ru1, LIU Run-hui1*, ZHANG Wei-dong1,3, HUANG Hong-lin2, ZHANG Yi5, HU Yao-hua1,4 and WANG Shu-ping1   

  1. 1. School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China;
    2. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, P. R. China;
    3. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, P. R. China;
    4. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, P. R. China;
    5. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P. R. China
  • 收稿日期:2010-02-05 修回日期:2010-04-26 出版日期:2011-01-25 发布日期:2011-01-04
  • 通讯作者: LIU Run-hui E-mail:lyliurh@126.com
  • 基金资助:

    Supported by the National Natural Science Foundation of China(No.30725045), the Foundation of Eleventh Five-Year-Plan of China(No.2008ZX09202-002), the Shanghai Leading Academic Discipline Project, China(No.B906) and the Scientific Foundation of Shanghai City, China(No.07DZ19702).

Qualitative and Quantitative Analysis of Alkaloids in Cortex Phellodendri by HPLC-ESI-MS/MS and HPLC-DAD

ZHU Shuang-lai1,2, DOU Sheng-shan1, LIU Xin-ru1, LIU Run-hui1*, ZHANG Wei-dong1,3, HUANG Hong-lin2, ZHANG Yi5, HU Yao-hua1,4 and WANG Shu-ping1   

  1. 1. School of Pharmacy, Second Military Medical University, Shanghai 200433, P. R. China;
    2. School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, P. R. China;
    3. School of Pharmacy, Shanghai Jiaotong University, Shanghai 200240, P. R. China;
    4. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350108, P. R. China;
    5. School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, P. R. China
  • Received:2010-02-05 Revised:2010-04-26 Online:2011-01-25 Published:2011-01-04
  • Contact: LIU Run-hui E-mail:lyliurh@126.com
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.30725045), the Foundation of Eleventh Five-Year-Plan of China(No.2008ZX09202-002), the Shanghai Leading Academic Discipline Project, China(No.B906) and the Scientific Foundation of Shanghai City, China(No.07DZ19702).

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摘要： A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectro- meter(HPLC-ESI-MS/MS) coupled with a photodiode array detector(HPLC-DAD) and principal component analysis(PCA) was applied to the qualitative and quantitative analyses of alkaloids in Cortex Phellodendri(CP) samples, and to the differentiation of two species of CP, Cortex Phellodendri Chinensis(CPC) and Cortex Phellodendri Amurensis(CPA). Twenty-two peaks appeared in the HPLC-MS base peak chromatogram of CP detected by the HPLC-ESI-MS/MS analysis, and the alkaloids were identified according to the MSn data, the known MS fragmentation rules and the literature data. Five alkaloids including berberine, palmatine, jatrorrhizine, phellodendrine and magnoflorine were simultaneously determinated by the HPLC-DAD. Berberine was the primary component in all CP samples, and the contents of berberine and palmatine were exploited to be two critical parameters for effective discrimination between the two species of CP. The average content of berberine in CPC(58.75 mg/g) was higher than that in CPA(9.16 mg/g), while the content of palmatine was less, only 0.25 mg/g in CPC and 4.19 mg/g in CPA. With the use of PCA, samples datasets were separated successfully into two different clusters corresponding to the two species, and berberine, palmatine, phellodendrine and magnoflorine contribute most to the above mentioned calssi- fying. The proposed method proved to be a useful tool in the quality control of Chinese herbal medicines.

关键词: Cortex Phellodendri, Alkaloid, HPLC-ESI-MS/MS, Quantitative analysis, Qualitative analysis, Principal component analysis

Abstract: A combined method of high performance liquid chromatograph-elecrtrospray-ionization mass spectro- meter(HPLC-ESI-MS/MS) coupled with a photodiode array detector(HPLC-DAD) and principal component analysis(PCA) was applied to the qualitative and quantitative analyses of alkaloids in Cortex Phellodendri(CP) samples, and to the differentiation of two species of CP, Cortex Phellodendri Chinensis(CPC) and Cortex Phellodendri Amurensis(CPA). Twenty-two peaks appeared in the HPLC-MS base peak chromatogram of CP detected by the HPLC-ESI-MS/MS analysis, and the alkaloids were identified according to the MSn data, the known MS fragmentation rules and the literature data. Five alkaloids including berberine, palmatine, jatrorrhizine, phellodendrine and magnoflorine were simultaneously determinated by the HPLC-DAD. Berberine was the primary component in all CP samples, and the contents of berberine and palmatine were exploited to be two critical parameters for effective discrimination between the two species of CP. The average content of berberine in CPC(58.75 mg/g) was higher than that in CPA(9.16 mg/g), while the content of palmatine was less, only 0.25 mg/g in CPC and 4.19 mg/g in CPA. With the use of PCA, samples datasets were separated successfully into two different clusters corresponding to the two species, and berberine, palmatine, phellodendrine and magnoflorine contribute most to the above mentioned calssi- fying. The proposed method proved to be a useful tool in the quality control of Chinese herbal medicines.

Key words: Cortex Phellodendri, Alkaloid, HPLC-ESI-MS/MS, Quantitative analysis, Qualitative analysis, Principal component analysis