Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

高等学校化学研究 ›› 2010, Vol. 26 ›› Issue (6): 955-957.

Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

ZHANG Di¹, GUO Lei¹, ZHU Na¹, SU Wei-heng¹, GUAN Xin-gang¹, YI Fei¹, HAO Feng¹, XIAO Ying-hong¹, LIU Jia², LIU Tian-zhou² and MA Tong-hui^1,2*

1. Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China;
2. The Second Hospital of Jilin University, Changchun 130041, P. R. China

收稿日期:2010-06-07 修回日期:2010-07-09 出版日期:2010-11-25 发布日期:2010-12-01
通讯作者: MA Tong-hui E-mail:math108@gmail.com
作者简介:MA Tong-hui. E-mail: math108@gmail.com
基金资助:
Supported by the National Basic Research Program of China(No.2009CB521908), the National Natural Science Foundation of China(Nos.30470405, 30570864, 30670477 and 30770493) and the National Natural Science Fund for Distinguished Young Scholars of China(No.30325011).

Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

ZHANG Di¹, GUO Lei¹, ZHU Na¹, SU Wei-heng¹, GUAN Xin-gang¹, YI Fei¹, HAO Feng¹, XIAO Ying-hong¹, LIU Jia², LIU Tian-zhou² and MA Tong-hui^1,2*

1. Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China;
2. The Second Hospital of Jilin University, Changchun 130041, P. R. China

Received:2010-06-07 Revised:2010-07-09 Online:2010-11-25 Published:2010-12-01
Contact: MA Tong-hui E-mail:math108@gmail.com
About author:MA Tong-hui. E-mail: math108@gmail.com
Supported by:
Supported by the National Basic Research Program of China(No.2009CB521908), the National Natural Science Foundation of China(Nos.30470405, 30570864, 30670477 and 30770493) and the National Natural Science Fund for Distinguished Young Scholars of China(No.30325011).

摘要/Abstract

摘要： We reported the expression and function of aquaglyceroporin AQP3 in mouse peripheral nervous system. AQP3 mRNA was identified in freshly isolated mouse sciatic nerve by RT-PCR. Immunofluorescence using AQP3 antibody localized the protein expression in the myelin sheathe of sciatic nerve fibers. Although primary morpholo- gical evaluation of sciatic nerves from AQP3-knockout and wildtype mice revealed no significant difference, biochemical analysis demonstrated remarkably decreased glycerol and ATP contents in freshly isolated AQP3-knockout sciatic nerve. The study indicates an important role of facilitated glycerol transport mechanism in peripheral nerve energy metabolism.

关键词: Aquaporin, Sciatic nerve myelin, Glycerol, ATP

Abstract: We reported the expression and function of aquaglyceroporin AQP3 in mouse peripheral nervous system. AQP3 mRNA was identified in freshly isolated mouse sciatic nerve by RT-PCR. Immunofluorescence using AQP3 antibody localized the protein expression in the myelin sheathe of sciatic nerve fibers. Although primary morpholo- gical evaluation of sciatic nerves from AQP3-knockout and wildtype mice revealed no significant difference, biochemical analysis demonstrated remarkably decreased glycerol and ATP contents in freshly isolated AQP3-knockout sciatic nerve. The study indicates an important role of facilitated glycerol transport mechanism in peripheral nerve energy metabolism.

Key words: Aquaporin, Sciatic nerve myelin, Glycerol, ATP

ZHANG Di, GUO Lei, ZHU Na, SU Wei-heng, GUAN Xin-gang, YI Fei, HAO Feng, XIAO Yi. Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve[J]. 高等学校化学研究, 2010, 26(6): 955-957.

ZHANG Di, GUO Lei, ZHU Na, SU Wei-heng, GUAN Xin-gang, YI Fei, HAO Feng, XIAO Ying-hong, LIU Jia, LIU Tian-zhou and MA Tong-hui*. Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve[J]. Chemical Research in Chinese Universities, 2010, 26(6): 955-957.

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Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

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